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Related Concept Videos

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

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One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
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Freeze-Dried Bacterial Cellulose-Based Point-of-Care Device for Antibiotic Susceptibility Testing (AST).

Chebiyyam Sarita1,2,3, Mudrika Khandelwal2, Suhanya Duraiswamy3

  • 1Center For Interdisciplinary Programs, Indian Institute of Technology Hyderabad, Hyderabad, Telangana, India.

Macromolecular Bioscience
|January 15, 2026
PubMed
Summary

This study introduces bacterial cellulose devices for rapid antibiotic susceptibility testing. These novel devices visually distinguish bacterial resistance within a single doubling time, addressing a key global health challenge.

Keywords:
antibiotic susceptibility testingbacterial cellulosecolorimetric detectionimage processingpoint‐of‐care diagnostics

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Area of Science:

  • Biomaterials Science
  • Microbiology
  • Medical Diagnostics

Background:

  • Antimicrobial resistance (AMR) poses a significant global health threat.
  • Current antibiotic susceptibility testing (AST) methods are time-consuming, often requiring 16-48 hours.
  • There is a critical need for rapid diagnostics to combat AMR effectively.

Purpose of the Study:

  • To develop and evaluate bacterial cellulose-based devices for rapid, colorimetric antimicrobial susceptibility profiling.
  • To assess the suitability of freeze-dried bacterial cellulose as a substrate for AST.
  • To demonstrate visual distinction of bacterial susceptibility and resistance within a single bacterial doubling time.

Main Methods:

  • Utilized freeze-dried bacterial cellulose with a nanofibrous, porous structure as a substrate.
  • Incorporated bacterial inoculum, antibiotics, and a halochromic dye into the bacterial cellulose device.
  • Evaluated moisture retention properties compared to traditional filter paper.
  • Employed K-means clustering for quantitative analysis of colorimetric outputs.

Main Results:

  • Bacterial cellulose devices exhibited superior moisture retention compared to filter paper.
  • Visual distinction between susceptibility (to ampicillin) and resistance (to nalidixic acid) was achieved within a single bacterial doubling time for E. coli and S. aureus.
  • The platform demonstrated effective accommodation of inoculum, antibiotics, and dye.

Conclusions:

  • Freeze-dried bacterial cellulose devices offer a promising platform for rapid antimicrobial susceptibility testing.
  • These devices enable visual and rapid determination of bacterial response to antibiotics.
  • The developed method significantly reduces the time required for AST, aiding in faster clinical decisions.