Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is bilateral laminectomy and fusion superior to total laminectomy and fusion in two-level lumbar spinal stenosis?

Frontiers in surgery·2026
Same author

TMEM216 inhibits breast cancer lung metastasis by modulating IGF1R-IRS4 signaling pathway.

Nature communications·2026
Same author

A Cascaded Classification-Regression Framework for Shear Strength Prediction of Cold-Formed Steel Screw Connections.

Materials (Basel, Switzerland)·2026
Same author

Quantitative Parameters of Contrast-Enhanced Ultrasonography (CEUS) Monitoring Ovarian Hemodynamics in Rats.

Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association·2026
Same author

Resistant Peanut Genotype Reprograms Rhizosphere Metabolism to Enhance Bacterial Wilt Suppression.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Global, regional, and national burden of clavicle, scapula, or humerus fracture in 204 countries and territories, 1990 to 2021: A systematic analysis from the Global Burden of Disease Study 2021.

Medicine·2026

Related Experiment Video

Updated: Jun 20, 2026

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration
12:21

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

Published on: November 26, 2015

18.3K

Rasd2 Knockout Exaggerates the Hearing Loss Phenotype of Tsc1-Deficient Mice.

Rui Ren1,2, Haiyue Xing1, Xiaoying Wang1

  • 1Shandong Provincial Key Laboratory of Development and Regeneration and Key Laboratory for Experimental Teratology of the Ministry of Education, School of Life Sciences, Shandong University, Qingdao, Shandong, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|January 15, 2026
PubMed
Summary

RASD2, a protein in the inner ear, does not affect hearing alone but worsens hearing loss when combined with TSC1 deficiency. This suggests RASD2 regulates mTOR activity and stereocilia morphology for normal hearing.

Keywords:
RASD2TSC1hair cellsinner earmTOR pathwaystereocilia

More Related Videos

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice
08:51

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Published on: May 10, 2019

12.4K
Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage
07:13

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

Published on: February 10, 2023

2.8K

Related Experiment Videos

Last Updated: Jun 20, 2026

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration
12:21

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

Published on: November 26, 2015

18.3K
Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice
08:51

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Published on: May 10, 2019

12.4K
Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage
07:13

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

Published on: February 10, 2023

2.8K

Area of Science:

  • Inner ear biology
  • Molecular genetics
  • Auditory neuroscience

Background:

  • Rasd2 (Ras-related Dexras1) is a striatum-enriched transcript implicated in mTOR signaling.
  • Rasd2 is also found in inner ear hair cells, but its function there is unknown.
  • TSC1 is a negative regulator of mTOR signaling, and its deficiency causes hearing loss.

Purpose of the Study:

  • To investigate the role of Rasd2 in the inner ear and its contribution to hearing.
  • To determine if Rasd2 interacts with TSC1 in regulating hair cell function and auditory perception.

Main Methods:

  • Generated Rasd2 knockout mice using CRISPR/Cas9.
  • Generated Rasd2/Tsc1 double knockout mice.
  • Analyzed hair cell stereocilia morphology and auditory function.
  • Assessed mTORC1 pathway activation in cochlear tissues.

Main Results:

  • Rasd2 knockout alone did not affect auditory function or stereocilia.
  • Rasd2 knockout exacerbated hearing loss in Tsc1-deficient mice.
  • Double knockout mice exhibited severe stereocilia defects and enhanced mTORC1 activation.

Conclusions:

  • RASD2 plays a critical role in maintaining stereocilia morphology and auditory function.
  • RASD2 likely regulates mTOR activity, contributing to hearing transduction.
  • The interaction between RASD2 and TSC1 is crucial for normal inner ear function.