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Updated: Jul 15, 2026

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
Published on: January 13, 2016
Protocol for dual spatial transcriptomic profiling of infected tissues
Hao Zhou1, Ankush Tyagi2, Maxine McClain2
1Department of Biological Sciences, College of Medicine and Health Sciences, Khalifa University, Abu Dhabi, United Arab Emirates; Center for Biotechnology (BTC), Khalifa University, Abu Dhabi, United Arab Emirates.
None:
Dual spatial transcriptomics analysis offers profiling of host- and pathogen-specific transcriptional patterns in infected tissues to establish pathoadaptation signatures and predict infection outcomes. Here, we present a protocol for tissue processing, imaging, selection of regions of interest, library preparation, sequencing, and analysis pipeline. This protocol has a potential application for the analysis of any infected tissue. For complete details on the use and execution of this protocol, please refer to Zhou et al.1.
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