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Related Experiment Video

Updated: Jan 17, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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Protocol for dual spatial transcriptomic profiling of infected tissues.

Hao Zhou1, Ankush Tyagi2, Maxine McClain2

  • 1Department of Biological Sciences, College of Medicine and Health Sciences, Khalifa University, Abu Dhabi, United Arab Emirates; Center for Biotechnology (BTC), Khalifa University, Abu Dhabi, United Arab Emirates.

STAR Protocols
|January 15, 2026
PubMed
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This summary is machine-generated.

This study introduces a dual spatial transcriptomics protocol to analyze host and pathogen gene expression in infected tissues. This method helps identify infection signatures and predict outcomes in various tissues.

Area of Science:

  • Microbiology
  • Genomics
  • Pathology

Background:

  • Dual spatial transcriptomics enables simultaneous profiling of host and pathogen transcriptional patterns in infected tissues.
  • Understanding these patterns is crucial for identifying pathoadaptation signatures and predicting infection outcomes.

Purpose of the Study:

  • To present a comprehensive protocol for dual spatial transcriptomics analysis of infected tissues.
  • To provide a standardized method applicable to various infected tissue types.

Main Methods:

  • The protocol covers tissue processing, imaging, region of interest selection, library preparation, and sequencing.
  • A detailed analysis pipeline is included for processing the generated data.

Main Results:

Keywords:
RNA-seqbioinformaticsgene expressiongenomicssequence analysissystems biology

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  • The protocol allows for detailed profiling of host- and pathogen-specific transcriptional patterns.
  • It facilitates the establishment of pathoadaptation signatures.

Conclusions:

  • The presented protocol is a valuable tool for studying host-pathogen interactions in infected tissues.
  • It has broad potential applications for analyzing diverse infected tissue samples.