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FUSION: A fast and uniform processing framework for whole-brain optical scattering tomography images
Chong Chen1,2,3, Peilin Gu1,2,3, Martin Villiger3,4
1Department of Radiology, Massachusetts General Hospital, Boston, MA 02129, USA.
None:
Coherent scattering imaging has seen substantial development in clinical ophthalmology and endoscopic microscopy owing to its high throughput, large dynamic range, and capability for label-free imaging. However, limited optical penetration has constrained its application to whole-organ imaging studies. Here, we propose a high-throughput coherent scattering imaging system assisted by tissue clearing, termed fuCAST, which enables whole-brain white-matter imaging. In parallel, we develop a high-throughput image reconstruction and uniform stitching platform, FUSION, specifically tailored for fuCAST datasets.
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