Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

4.7K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
4.7K
IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

1.9K
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
1.9K
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

2.1K
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
2.1K
IR Spectrometers01:25

IR Spectrometers

2.4K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
2.4K
IR Spectrum01:19

IR Spectrum

2.0K
When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0%...
2.0K
IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

IR and UV–Vis Spectroscopy of Aldehydes and Ketones

7.3K
Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antifungal resistance: from classical mechanisms to the expanding role of acetyltransferases.

Journal of biomedical science·2026
Same author

Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for <i>Sporothrix</i> species using CLSI broth microdilution".

Antimicrobial agents and chemotherapy·2026
Same author

Why intrinsic reduced susceptibility in rare fungal pathogens should be differentiated from intrinsic resistance: an ISHAM conceptual framework and narrative review.

Antimicrobial agents and chemotherapy·2026
Same author

Label-Free Nanostructured Biosensing Platform Based on Depolarized Dynamic Light Scattering for Rapid and Portable Detection of Immunoglobulins in Complex Biological Samples.

ACS omega·2026
Same author

Efficacy Evaluation of Luliconazole-Loaded Nanostructured Lipid Carriers in Treatment-Resistant Dermatophytosis: A Randomized Clinical Trial.

Mycoses·2026
Same author

Neonatal Candida infections in Brazil: study on virulence and antifungal susceptibility with predominance of non-albicans Candida.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]·2026

Related Experiment Video

Updated: Jan 18, 2026

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.8K

Infrared Spectroscopy as a Promising Tool for Diagnosing and Typing Human Pathogenic Fungi.

Anthony G J Medeiros1, Ayrton L F Nascimento2, Luana Rossato3

  • 1Centro de Biociências, Universidade Federal Do Rio Grande Do Norte, Natal, Brazil.

Mycoses
|January 16, 2026
PubMed
Summary

Infrared (IR) spectroscopy offers a rapid, cost-effective diagnostic tool for fungal infections, improving identification and potentially guiding treatment, especially in resource-limited settings.

Keywords:
Biotyperfungal identificationinfrared radiationmedical mycology

More Related Videos

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

4.5K
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
07:58

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Published on: June 19, 2013

13.4K

Related Experiment Videos

Last Updated: Jan 18, 2026

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.8K
Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

4.5K
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
07:58

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Published on: June 19, 2013

13.4K

Area of Science:

  • Medical Mycology
  • Spectroscopy
  • Infectious Diseases

Background:

  • Fungal infections pose a significant global health threat, particularly to immunocompromised individuals.
  • Current diagnostic methods (culture, molecular) are often slow, costly, or require extensive infrastructure.
  • There is a critical need for rapid, accessible, and cost-effective fungal diagnostic tools.

Purpose of the Study:

  • To review the application of infrared (IR) spectroscopy in medical mycology for diagnosing fungal infections.
  • To examine the chemical principles and advancements in IR-based techniques (MIR, NIR) for fungal identification.
  • To assess the potential of IR spectroscopy in improving clinical management and patient outcomes.

Main Methods:

  • Review of existing literature on IR spectroscopy applications in medical mycology.
  • Exploration of mid-infrared (MIR) and near-infrared (NIR) spectroscopy techniques.
  • Analysis of the role of multivariate analysis and machine learning in enhancing diagnostic accuracy.

Main Results:

  • IR spectroscopy enables rapid, cost-effective, and reagent-free identification of human pathogenic fungi.
  • Studies show accurate identification and typing of major fungal pathogens using IR spectroscopy.
  • IR spectroscopy can provide insights into antifungal resistance and aid in outbreak tracking.

Conclusions:

  • IR spectroscopy is a promising diagnostic strategy for fungal infections, especially in resource-limited settings.
  • Advancements in data analysis have significantly improved the accuracy of IR-based diagnostics.
  • Standardization and database expansion are key for wider clinical adoption, potentially transforming fungal infection management.