Detection of PD-1 and CD28 Expression in Lymphocytes by Flow Cytometry

Area of Science:

• Immunology
• Biotechnology
• Clinical Diagnostics

Background:

• Immune checkpoint analysis is crucial for cancer immunotherapy research, immune microenvironment exploration, and biomarker discovery.
• Programmed Death 1 (PD-1) and CD28 are key T cell surface receptors regulating immune responses.
• Accurate detection of immune checkpoints like PD-1 and CD28 on lymphocytes is vital for understanding immune responses and guiding cancer immunotherapy.

Purpose of the Study:

• To develop and optimize a flow cytometry-based method for quantitative assessment of PD-1 and CD28 expression on lymphocytes.
• To establish and validate a detailed protocol for immune checkpoint detection using the CYTEK NL-CLC flow cytometer.

Main Methods:

• Utilized the CYTEK NL-CLC flow cytometer for quantitative detection of PD-1 and CD28 on lymphocytes.
• Developed and validated a flow cytometry protocol, assessing key performance parameters.

Main Results:

• The developed method demonstrated high sensitivity and specificity for immune checkpoint detection.
• Validation metrics including precision, linearity, stability, reference interval, and accuracy met acceptable criteria.
• Results are approved for clinical testing, indicating the method's readiness for application.

Conclusions:

• The CYTEK NL-CLC flow cytometer provides a reliable platform for immune checkpoint analysis.
• This method supports cancer immunotherapy workflows and personalized medicine strategies.
• The validated assay is suitable for both clinical and research settings.