Related Experiment Video
Updated: Jan 18, 2026

Safety Precautions and Operating Procedures in an ABSL-4 Laboratory: 1. Biosafety Level 4 Suit Laboratory Suite Entry and Exit Procedures
Published on: October 3, 2016
Long-term retention following best practices instruction for proper donning and doffing of personal protective
Terrie Vasilopoulos1, Cameron R Smith2, Amanda M Frantz2
1Department of Anesthesiology, University of Florida College of Medicine, Gainesville, FL; Department of Orthopaedic Surgery and Sports Medicine, University of Florida College of Medicine, Gainesville, FL.
Background:
The recent history of viral global epidemics highlights the importance of teaching the proper use of personal protective equipment (PPE) for personal safety and infection prevention. The present study evaluated long-term retention of knowledge for proper donning and doffing of PPE following face-to-face and video instruction.
Methods:
Three cohorts of residents were examined, with long-term retention assessment occurring in subsets of each cohort at least 7 months following initial training. Without instruction, the anesthesiology residents donned appropriate PPE and then sprayed with Glo Germ before doffing to identify areas of contamination. Following instruction, the process was repeated both immediately post-training and at long-term follow-up.
Results:
Twenty-three participants completed long-term assessment. Overall donning compliance from post-training to long-term retention did not change significantly (mean difference = -4.5%; 95% CIs: -10.7%, 1.7%; P = .105); however, doffing compliance decreased significantly (mean difference = -20.7%; 95% CIs: -28.1%, -13.4%; P < .001). Contamination rates of multiple sites increased by 17.4% (P = .248), though did not reach statistical significance.
Conclusions:
At long-term follow-up, there was a higher retention of proper donning with a decay of knowledge with doffing. It is important to implement educational maintenance programs, not single courses, for healthcare workers to prevent contamination during these common, highly virulent epidemics.
Related Concept Videos
PPE Use in Healthcare Settings I: Donning
PPE Use in Healthcare Settings II: Doffing
Personal Protective Equipment
Standard Precaution
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...
Tracheostomy Care II: Procedure
Step 1: Perform hand hygiene, and put on personal protective equipment: gown, gloves, mask...
Administering Oxygen by Mask
Administering oxygen by mask is a common nursing intervention that provides supplemental oxygen to patients with respiratory distress or chronic lung conditions. This procedure involves delivering oxygen at a specified rate through a face mask connected to an oxygen source.
Equipment
The equipment necessary for this procedure includes:

