Anti-inflammatory polyketides produced by Antarctic-derived fungus Aspergillus ochraceopetaliformis SCSIO 05702
Meng-Jing Cong1, Xue Ren2, Zi-Yi Wu1
1Guangdong Key Laboratory of Marine Materia Medica, State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
Five undescribed compounds (1-5), together with four known compounds (6-9) were isolated from the Antarctic-derived fungus Aspergillus ochraceopetaliformis SCSIO 05702. Their structures were elucidated by the nuclear magnetic resonance spectrum (NMR), mass spectrometry (MS). In addition, the absolute configurations of these compounds were determined by the combination of ECD and DP4+ calculations, chiral-phase HPLC analysis, Mosher's ester analysis and Mo2(OAc)4 induced circular dichroism method. Among them, 5 and 6 exhibited potent anti-inflammatory responses induced by LPS in RAW264.7 cells. Specifically, 5 and 6 were able to markedly inhibit the production of NO and pro-inflammatory cytokines including IL-6, TNF-α, and MCP-1 in RAW264.7 cells exposed to 0.1 μg/mL LPS. Moreover, they effectively upregulated the anti-inflammatory cytokines, such as IL-4, IL-10, and Arg-1 gene expression levels impaired by LPS without obvious cytotoxicity.
