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Updated: Jan 20, 2026

Biofilm Removal Using Carbon Dioxide Aerosols without Nitrogen Purge
Published on: November 6, 2016
Exploring efficient mainstream nitrogen removal by partial nitrification/anammox under limited COD condition in a
Lisheng Wang1, Congcong Zhang1, Xiaofeng Kang1
1State Key Laboratory of Regional Environment and Sustainability, School of Environment, School of Environment, Tsinghua University, Beijing, China.
Abstract:
The integration of partial nitrification/anammox (PN/A) into membrane-aerated biofilm reactor (MABR) is a promisingly energy-efficient and high-efficiency technology for nitrogen removal. For the first time, this study investigated the practicability and stability in the combined pilot-scale MABR unit and anaerobic ammonium-oxidizing bacteria (AnAOB)-carriers unit to remove nitrogen under limited COD condition by PN/A. About 83% of NH₄⁺-N removal efficiency was realized with the influent flow rate of 2.4 m3/d (i.e. the hydraulic retention time of 12 h) by nitrification in MABR system before the inoculation of AnAOB. The free nitrous acid (FNA) enhanced strategy showed remarkable NOB inactivation and stable PN. After the inoculation of AnAOB, the total nitrogen removal efficiency was 78% with the influent flow rate of 1.2 m3/d, and the percentage of nitrogen removal by PN/A accounted for 55%. The optimizing operational conditions suggest that for an effective strategy to balance water quality and the nitrogen removal rate of the MABR, a proper biofilm thickness controlling strategy is recommend for future practice. The obtained results would further improve our perspectives on NOB inhibition in the pilot-scale MABR system and shed light on the future practical application for a sustainable wastewater treatment by PN/A technology.
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