Host-centric approach toward increased recombinant protein solubility

This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called...

This video describes a colorimetric method to quantify the soluble protein content in plant tissues. The method uses Coomassie Brilliant Blue G-250 dye, which interacts with the soluble protein to form a blue-colored stable protein-dye complex, proportional to the soluble protein content in the...

This is a method to generate “scarless” recombinant vaccinia viruses using host-range selection and visual identification of recombinant...

Solubility equilibria are established when the dissolution and precipitation of a solute species occur at equal rates. These equilibria underlie many natural and technological processes, ranging from tooth decay to water purification. An understanding of the factors affecting compound solubility is, therefore, essential to the effective management of these processes. This section applies previously introduced equilibrium concepts and tools to systems involving dissolution and precipitation.
The...

Developing biotinylatable fusion proteins has many potential applications in various fields of research. Recombinant protein engineering is a straight forward procedure that is cost-effective, providing high yields of custom-designed...

Overview