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Updated: Jan 20, 2026

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
Published on: September 21, 2019
Covalent fluorescent probes for 2-arachidonoylglycerol metabolic pathways
Nick D F Puijmbroeck1, Mario van der Stelt1
1Department of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
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Covalent fluorescent probes have emerged as versatile chemical tools to visualise active enzymes in complex biological systems. When tailored for specific applications, ranging from activity-based protein profiling for drug development to high spatiotemporal resolution imaging of enzymatic activities, these probes provide unique insights into rapid lipid metabolism. The recent development of reverse-designing optimised inhibitors into probes, together with improved analytical techniques, is rapidly advancing our ability to resolve enzyme function in great detail. In this mini-review, we summarise the current landscape of covalent fluorescent probes for the 2-arachidonoylglycerol (2-AG) metabolising enzymes (diacylglycerol lipase, monoacylglycerol lipase, ABHD6 and ABHD12), highlighting the discoveries they have enabled and discussing strategies to address gaps where probes remain lacking.
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