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RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

A highly sensitive ribozyme-based assay, applicable to high-throughput screening of chemicals targeting the unique process of RNA editing in trypanosomatid pathogens, is described in this paper. Inhibitors can be used as tools for hypothesis-driven analysis of the RNA editing process and ultimately as...

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.

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Genome Editing

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A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage,...

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Related Experiment Video

Updated: Jan 20, 2026

RNA Editing: Post-transcriptional Base Insertion, Deletion or Modification

RNA Editing: Post-transcriptional Base Insertion, Deletion or Modification

Multi-Tissue Genetic Regulation of RNA Editing in Pigs.

Xiangchun Pan¹, Wentao Gong¹, Xiaodian Cai¹

¹State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|January 18, 2026

Summary

This summary is machine-generated.

This study maps RNA editing quantitative trait loci (edQTLs) in pigs, revealing their crucial role in complex traits and heritability. It establishes RNA editing as a key link between genetic variation and phenotypic diversity in pigs.

Keywords:

PigGTEx RNA editing RNA editing quantitative trait loci RNA‐seq SNP

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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

Related Experiment Videos

Last Updated: Jan 20, 2026

RNA Editing: Post-transcriptional Base Insertion, Deletion or Modification

RNA Editing: Post-transcriptional Base Insertion, Deletion or Modification

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

Area of Science:

Genomics
Molecular Biology
Agricultural Science

Background:

RNA editing contributes to transcriptomic variation and complex trait regulation.
The genetic determination of RNA editing in pigs is not well understood.

Purpose of the Study:

To construct a comprehensive RNA editome and map cis-editing quantitative trait loci (edQTLs) in pigs.
To investigate the regulatory role of RNA editing in complex traits and its relationship with gene expression and splicing.
To explore conserved editing events between humans and pigs.

Main Methods:

Utilized 5457 PigGTEx RNA-seq samples across 34 porcine tissues.
Constructed a comprehensive RNA editome and mapped 49,614 cis-edQTLs.
Performed colocalization analyses and human-pig comparative analysis.

Main Results:

Identified 49,614 cis-edQTLs, with over 32% acting independently of expression or splicing QTLs.
Found significant enrichment of edQTLs in GWAS loci for 33 complex traits, showing a superior contribution to heritability for traits like growth and reproduction.
Discovered diverse causal mechanisms, including edQTL-specific effects and pleiotropic effects modulating RNA editing, splicing, and gene expression.
Identified hundreds of evolutionarily conserved editing events linked to neurological pathways.

Conclusions:

RNA editing represents a distinct regulatory layer influencing complex traits in pigs.
edQTLs are a significant driver of heritability for traits such as growth and reproduction.
This study provides the first comprehensive porcine RNA editome and edQTL map, highlighting RNA editing's role in linking genetic variation to phenotypic diversity.