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Related Experiment Video

Updated: Jan 20, 2026

Acinar-to-Ductal Metaplasia Induction: A Method to Study Acinar Cell to Ductal Cell Differentiation in 3D Ex Vivo Culture

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Published on: April 30, 2023

Modulating O-GlcNAcylation Alters Salivary Acinar Cell Differentiation.

Elina Pokharel¹, Tae-Young Kim^1,2, Yam Prasad Aryal^1,3

¹Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, South Korea.

Journal of Cellular Physiology

|January 19, 2026

Summary

This summary is machine-generated.

O-GlcNAcylation, regulated by O-GlcNAc transferase (OGT), is crucial for salivary gland development. Inhibiting OGT disrupts acinar cell differentiation and morphogenesis by altering key signaling pathways.

Keywords:

OGT OSMI‐1 O‐GlcNAcylation acinar cell differentiation salivary gland

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Last Updated: Jan 20, 2026

Acinar-to-Ductal Metaplasia Induction: A Method to Study Acinar Cell to Ductal Cell Differentiation in 3D Ex Vivo Culture

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Published on: November 17, 2023

Area of Science:

Biochemistry
Developmental Biology
Cellular Biology

Background:

O-GlcNAcylation is a dynamic post-translational modification regulating diverse cellular functions.
O-GlcNAc transferase (OGT) and O-GlcNAc exhibit specific localization during mouse salivary gland development, suggesting a role in acinar cell differentiation.

Purpose of the Study:

To investigate the role of OGT-mediated O-GlcNAcylation in mouse submandibular gland morphogenesis and acinar cell differentiation.
To elucidate the molecular mechanisms underlying OGT's function in salivary gland development.

Main Methods:

In vitro cultivation of mouse submandibular glands.
Inhibition of OGT using OSMI-1 and OGT-targeting siRNA.
Assessment of morphological changes via histology and immunohistochemistry.
Molecular analysis using Western blot and RT-qPCR.

Main Results:

OGT inhibition impaired terminal bud morphogenesis and disrupted acinar cell differentiation.
OSMI-1 treatment altered the expression of key signaling molecules (Sox9, Sox10, E-cadherin, Mist1) involved in acinar cell differentiation.
Changes in cytokeratin expression (CK14, CK18) indicated altered ductal morphology.

Conclusions:

OGT-mediated O-GlcNAcylation is essential for proper salivary gland morphogenesis.
O-GlcNAcylation regulates the post-translational modification of signaling molecules critical for functional acinar cell differentiation.