Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

10.1K
The measurement protocol and data analysis procedure are given for obtaining transverse coherence of a synchrotron radiation X-ray source along four directions simultaneously using a single 2-D checkerboard phase grating. This simple technique can be applied for complete transverse coherence characterization of X-ray sources and X-ray...
10.1K
Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation07:54

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

9.8K
Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.
9.8K
Reflection and Refraction13:59

Reflection and Refraction

39.8K
Source: Derek Wilson, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA
Light travels at different speeds depending on the material through which it is propagating. When light travels from one material to another, it will either slow down or speed up. In order to conserve energy and momentum, the light must change the direction in which it propagates. This bending of light is known as refraction. Some fraction of...
39.8K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

15.3K
This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand...
15.3K
Tree Core Analysis with X-ray Computed Tomography06:56

Tree Core Analysis with X-ray Computed Tomography

1.6K
Here we show how to process tree cores with an X-ray computed tomography toolchain. Except for chemical extraction for some purposes, no further physical lab treatment is needed. The toolchain can be used for biomass estimations, for obtaining MXD/tree-ring width data as well as for obtaining quantitative wood anatomy data.
1.6K
Characterization Of Multi-layered Fish Scales (Atractosteus spatula) Using Nanoindentation, X-ray CT, FTIR, and SEM10:06

Characterization Of Multi-layered Fish Scales (Atractosteus spatula) Using Nanoindentation, X-ray CT, FTIR, and SEM

15.5K
This paper presents the methods used for probing spatially correlated chemical, structural, and mechanical properties of the multilayered scale of Atractosteus spatula (A. spatula) using nanoindentation, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and X-ray computed tomography (X-ray CT). The experimental results have been used to investigate the design principles of protective biological...
15.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adsorption and Sulfur-Selective Photooxidation of Cysteine on Anatase TiO<sub>2</sub>(101).

Journal of the American Chemical Society·2026
Same author

Cation-Surface Interactions During Electrocatalytic Hydrogen Evolution Probed by Surface X‑ray Diffraction.

ACS physical chemistry Au·2026
Same author

Atomic Imaging of Ion-Triggered Flexibility and Local Electric Field Response in Zeolite Rings.

Journal of the American Chemical Society·2026
Same author

Revealing formic acid adsorption geometries on magnetite (001) and (111) surfaces by IRRAS line shape analysis.

Physical chemistry chemical physics : PCCP·2026
Same author

Platinum oxide formation under oxygen evolution reaction conditions.

Nature communications·2026
Same author

3D electron diffraction-the missing slice completing nanoscale analysis of organic solar cells in TEM.

Nature communications·2026

Related Experiment Video

Updated: Jan 20, 2026

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

10.1K

X-ray reflectivity from micrometre-scaled surfaces using nanobeams.

Vedran Vonk1, Steffen Tober2, Steven J Leake3

  • 1Centre for X-ray and Nano Science CXNS Deutsches Elektronen-Synchrotron DESY Notkestraße 85 22607 Hamburg Germany.

Journal of Applied Crystallography
|January 19, 2026
PubMed
Summary
This summary is machine-generated.

Precise alignment is key for nanoscale grazing-incidence X-ray measurements. This study introduces a 1D trajectory scan method to maintain sample position in the X-ray beam, improving reflectivity data accuracy.

Keywords:
X-ray nanobeamsX-ray reflectivity

More Related Videos

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
07:54

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

Published on: March 12, 2015

9.8K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.3K

Related Experiment Videos

Last Updated: Jan 20, 2026

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

10.1K
Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
07:54

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

Published on: March 12, 2015

9.8K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.3K

Area of Science:

  • Materials Science
  • Surface Science
  • Nanotechnology

Background:

  • Accurate sample alignment is critical for nanoscale grazing-incidence X-ray measurements.
  • Maintaining a consistent beam-sample interaction is challenging due to systematic errors and sample movement.

Purpose of the Study:

  • To develop and demonstrate a method for keeping a specific point of interest on a surface within the X-ray beam during reflectivity measurements.
  • To address challenges in aligning samples at the nanometer scale for X-ray analysis.

Main Methods:

  • A 1D trajectory scan technique was employed, varying the angle of incidence (θ), detector angle (2θ), and sample position along one axis.
  • X-ray reflectivity measurements were performed on a 10 × 10 µm gold (Au) island using a 90 nm beam.

Main Results:

  • The 1D trajectory scan successfully maintained the sample's position within the X-ray beam.
  • Data analysis accounted for the angle-dependent X-ray beam footprint on both the sample and surrounding support.

Conclusions:

  • The developed 1D trajectory scan method enhances the reliability of nanoscale X-ray reflectivity measurements.
  • This technique is valuable for accurate surface analysis when precise sample positioning is difficult.