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Coronoid-Temporalis Pedicled Flap for Orbital Floor Defect Reconstruction06:32

Coronoid-Temporalis Pedicled Flap for Orbital Floor Defect Reconstruction

This protocol presents a novel coronoid-temporalis pedicled flap (CTPF) technique for reconstructing small-to-medium orbital floor defects. CTPF avoids microvascular risks while maintaining biomechanical stability. Clinical cases demonstrate effective structural restoration, reduced complications, and preserved facial symmetry, offering a viable alternative to traditional grafts or alloplastic materials.
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The Superficial Inferior Epigastric Artery Fascia Flap for Nerve Reconstruction in Rabbits12:01

The Superficial Inferior Epigastric Artery Fascia Flap for Nerve Reconstruction in Rabbits

This protocol presents a step-by-step instruction for harvesting the superficial inferior epigastric artery fascia (SIEF) flap and wrapping it around the reconstructed peroneal nerve defect in rabbits. This surgical addition of vascularization to the tissue bed of nerve grafts is used to improve regeneration outcomes.
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Donor Posterior Atrial Flap Rotation for Left Atrial Cuff Reconstruction in Lung Transplantation07:28

Donor Posterior Atrial Flap Rotation for Left Atrial Cuff Reconstruction in Lung Transplantation

This protocol describes a new method for effectively reconstructing donor atrial cuff anterior wall defects during lung transplantation through the rotation of the donor posterior atrial flap.
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Engineered Vascularized Muscle Flap08:18

Engineered Vascularized Muscle Flap

To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.
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High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay10:30

High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay

The island assay is a relatively new, cost-effective assay that can be used to evaluate the basic locomotor behavior of Drosophila melanogaster. This manuscript describes algorithms for automatic data processing and objective quantification of island assay data, making this assay a sensitive, high-throughput readout for large genetic or pharmacological...
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3D Planning and Printing of Patient Specific Implants for Reconstruction of Bony Defects08:15

3D Planning and Printing of Patient Specific Implants for Reconstruction of Bony Defects

This protocol describes the use of 3D planning and printing for reconstruction of bony defects. We use segmentation tools to create 3D models followed by 3D design software to create patient specific implants for reconstruction purposes concomitant to ablative surgery or as a second...
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