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Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor15:19

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Microbial sulfate reduction is a process of great importance in environmental biotechnology. The success of the sulfidogenic reactors depends among other factors on the microbial composition of the sludge. Here, we present a protocol to develop sulfidogenic sludge from hydrothermal vents sediments in a UASB reactor for reductive dechlorination...
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Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)08:09

Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)

Here, protocols for performing microfocus X-ray computed tomography (microCT) imaging of three marine invertebrate animals are explained in detail. This study describes steps such as sample fixation, staining, mounting, scanning, image reconstruction, and data analyses. Suggestions on how the protocol can be adjusted for different samples are also...
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Sediment Core Extrusion Method at Millimeter Resolution Using a Calibrated, Threaded-rod06:06

Sediment Core Extrusion Method at Millimeter Resolution Using a Calibrated, Threaded-rod

An extrusion method using a calibrated threaded-rod is presented, which allows for mm scale subsampling of aquatic sediment cores. Millimeter-scale sampling is necessary to fully characterize recent event stratigraphy in sediment...
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Soxhlet Extraction of Lipid Biomarkers from Sediment08:04

Soxhlet Extraction of Lipid Biomarkers from Sediment

Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst
Every lab needs standards that track the performance, accuracy, and precision of its instruments over time to ensure a measurement made today is the same as a measurement made a year from now (Figure 1). Because standards must test the performance of instruments over a long period of time, large volumes of the standards are often required. Many chemical standards can be purchased from retail scientific companies, like...
19.6K
Sonication Extraction of Lipid Biomarkers from Sediment07:24

Sonication Extraction of Lipid Biomarkers from Sediment

Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst
The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...
12.8K
Selective Enrichment and Isolation of Oil-Degrading Marine Bacteria from Seawater Samples03:07

Selective Enrichment and Isolation of Oil-Degrading Marine Bacteria from Seawater Samples

Source: Villela, H. D. M., et al. Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation. J. Vis. Exp. (2019)This video demonstrates the selective isolation of oil-degrading marine bacteria by enriching seawater samples on oil-based media, enabling the recovery of pure strains with potential applications in crude oil bioremediation and environmental...
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