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Production and Aerosol-Based Deposition of Bacillus subtilis Spores03:37

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Source: Fuchs, F. M., et al. Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy. J. Vis. Exp. (2017)This video demonstrates the production, purification, and aerosol-based deposition of Bacillus subtilis spores to create a uniform monolayer, enabling downstream applications such as microscopy-based analysis of spore germination, viability, or stress...
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Related Experiment Video

Updated: Jan 20, 2026

Production and Aerosol-Based Deposition of Bacillus subtilis Spores
03:37

Production and Aerosol-Based Deposition of Bacillus subtilis Spores

Published on: October 30, 2025

294

Novel Thermostable α-Amylase from Bacillus subtilis: Molecular Characterization, Optimization, and Docking-Based

Shazeen Shoaib1, Shumaila Naz2, Iram Manzoor1

  • 1Department of Biosciences, Quaid Campus, University of Wah, Wah Cantt, 47040, Pakistan.

The Protein Journal
|January 19, 2026
PubMed
Summary

Researchers identified a novel alpha-amylase from Bacillus subtilis (S4) with high activity and stability. Molecular and in silico analyses, including docking, revealed its potential for industrial applications like starch processing.

Keywords:
Alpha-amylaseBacillus subtilisHomology modelingIn silicoMolecular docking

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Last Updated: Jan 20, 2026

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Area of Science:

  • Biotechnology
  • Enzymology
  • Molecular Biology

Background:

  • Bacterial alpha-amylases are crucial enzymes with broad industrial applications.
  • There is a continuous need for novel alpha-amylases with improved properties for various biotechnological processes.

Purpose of the Study:

  • To isolate and characterize a novel alpha-amylase-producing bacterium.
  • To determine the enzyme's substrate specificity and binding interactions using molecular docking.

Main Methods:

  • Isolation and identification of amylolytic bacteria (Bacillus subtilis S4).
  • Enzyme characterization (optimal temperature, pH, time).
  • Gene sequencing, protein analysis, homology modeling, and molecular docking.

Main Results:

  • Bacillus subtilis S4 exhibited high amylolytic activity (63.68 U/ml).
  • Optimal activity at 40°C and pH 7.0.
  • Molecular docking showed highest affinity for amylopectin (-7.2 kcal/mol), identifying key catalytic residues.

Conclusions:

  • The novel alpha-amylase from B. subtilis S4 possesses desirable industrial traits like stability and thermotolerance.
  • It is a promising candidate for cost-effective starch processing and other biotechnological applications.