Related Experiment Video
Updated: Jan 21, 2026

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods
Published on: December 23, 2013
A Modular Method for Rapidly Prototyping Targeted Gas Vesicle Protein Nanoparticles
Reid Vassallo1, Bill Ling2, Ernesto Criado-Hidalgo2,3
1School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Abstract:
Gas vesicles (GVs) are air-filled protein nanoparticles that are proving to be useful in a number of biomedical applications. We hypothesized that it could be possible to develop a modular method for creating rapidly prototyped GVs by modifying their surface chemistry to include targeting peptides in an orientation-specific manner. Here, we describe a modular method to create targeted GVs using His-tagged antibody fragments, ensuring that the antibody fragments are connected to the GV in an orientation-specific manner. This is achieved via the functionalization of the GVs with the nickel-nitrilotriacetic acid (Ni-NTA) group. First, we validated that these functionalized GVs can bind His-tagged green fluorescent protein and characterized the particle size and surface charge of functionalized GVs. Then, GVs targeted to prostate-specific membrane antigen (PSMA) using a minibody were validated using a knockout validation in vitro.
More Related Videos
Related Concept Videos
Concepts and Prototypes
The brain organizes this information using concepts, which are mental categories grouping linguistic data,...
Gas Exchange and Transport
Kinetic Molecular Theory and Gas Laws Explain Properties of Gas Molecules
Gas Laws: Boyle's, Gay-Lussac, Charles', Avogadro's, and Ideal Gas Law
Gas Chromatography: Introduction
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a...
Ideal Gas Equation

