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Related Experiment Video

Updated: Jan 21, 2026

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

ROS-responsive self-immolative polymeric prodrug for nitrosative stress-mediated cancer therapy.

Anup Dey¹, Jeongjin Lee¹, Minsung Park²

¹School of Chemical Engineering, College of Engineering, Sungkyunkwan University (SKKU), Seobu-ro 2066, Jangan-gu, Suwon 16419, Republic of Korea.

Journal of Controlled Release : Official Journal of the Controlled Release Society

|January 19, 2026

Summary

This summary is machine-generated.

This study introduces a novel polymer prodrug that releases nitric oxide (NO) and scavenges glutathione in tumors. This targeted approach shows potent anticancer effects and minimal toxicity, offering a new redox-responsive therapy.

Keywords:

Cancer treatment Glutathione depletion Nitric oxide Polymeric prodrug Reactive oxygen species Self-immolative polymer

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Production and Detection of Reactive Oxygen Species ROS in Cancers

Production and Detection of Reactive Oxygen Species ROS in Cancers

Published on: November 21, 2011

Area of Science:

Biomedical Engineering
Cancer Therapeutics
Drug Delivery Systems

Background:

Nitric oxide (NO) is a promising redox regulator for cancer therapy.
Conventional NO donors face limitations due to short half-lives and poor tumor specificity.
Reactive oxygen species (ROS) are elevated in the tumor microenvironment, presenting a therapeutic target.

Purpose of the Study:

To develop a ROS-responsive, self-immolative polymeric prodrug (G-g-PSIP) for targeted cancer therapy.
To investigate the prodrug's mechanism of action, including NO release, glutathione scavenging, and ROS responsiveness.
To evaluate the antitumor efficacy and safety of G-g-PSIP in vitro and in vivo.

Main Methods:

Synthesis and characterization of the G-g-PSIP polymeric prodrug.
In vitro cytotoxicity assays using CT26 cancer cells and L929 normal cells.
Assessment of intracellular glutathione depletion, NO generation, and peroxynitrite formation.
In vivo antitumor efficacy studies in CT26 tumor-bearing mice.

Main Results:

G-g-PSIP demonstrated dose-dependent cytotoxicity against CT26 cancer cells with minimal toxicity to normal cells.
The prodrug effectively scavenged glutathione and generated NO in response to elevated ROS.
Cascade-amplified depolymerization led to peroxynitrite accumulation, inducing nitrosative stress and ferroptosis-like cell death.
Systemic administration of G-g-PSIP achieved significant antitumor efficacy in vivo.

Conclusions:

G-g-PSIP is a potent ROS-responsive polymeric prodrug for targeted cancer therapy.
The synergistic effects of GSH depletion and NO/peroxynitrite generation induce ferroptosis-like cell death.
This redox-responsive platform holds promise for developing novel cancer treatments.