Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Structure01:23

RNA Structure

78.9K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
78.9K
RNA Structure01:19

RNA Structure

7.2K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
7.2K
Experimental Determination of Chemical Formula02:37

Experimental Determination of Chemical Formula

46.4K
The elemental makeup of a compound defines its chemical identity, and chemical formulas are the most concise way of representing this elemental makeup. When a compound’s formula is unknown, measuring the mass of its constituent elements is often the first step in determining the formula experimentally.
46.4K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

3.3K
3.3K
Chemical Stoichiometry and Gases: Using Ideal Gas Law to Determine Moles03:12

Chemical Stoichiometry and Gases: Using Ideal Gas Law to Determine Moles

29.4K
Chemical stoichiometry describes the quantitative relationships between reactants and products in chemical reactions.
29.4K
RNA Stability01:53

RNA Stability

35.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AND-Logic-Gated Aptamer Switch for Precise Targeting and Regulation of RNA G-Quadruplexes.

Angewandte Chemie (International ed. in English)·2026
Same author

MoRNiNG: A Database of RNA Modification Sites Associated with RNA Secondary Structure Dynamics.

Genomics, proteomics & bioinformatics·2025
Same author

Unusual "mesoionic" N^S biscyclometallated iridium(iii) polypyridine complexes as photosensitisers for photodynamic therapy and type II immunogenic cell death inducers.

Chemical science·2025
Same author

L-RNA Aptamer-Based Tools for G-Quadruplex Structure: Identification, Characterization, and Application.

Accounts of chemical research·2025
Same author

Differential Evolution of CDS and UTR Non-canonical RNA G-quadruplex Structures in Eukaryotic Transcriptomes.

Genomics, proteomics & bioinformatics·2025
Same author

TDP-43 binds to RNA G-quadruplex structure and regulates mRNA stability and translation.

Nucleic acids research·2025

Related Experiment Video

Updated: Jan 22, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

5.1K

Recent Advances in Chemical Probing Strategies for RNA Structure Determination In Vivo.

Maryana Yarshova1, Jieyu Zhao1, Chun Kit Kwok1,2

  • 1Department of Chemistry and State Key Laboratory of Marine Environmental Health, City University of Hong Kong, Hong Kong SAR, China.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|January 20, 2026
PubMed
Summary

Studying RNA structure in cells is challenging. New chemical probing methods and sequencing technologies now reveal the RNA structurome in its native cellular environment.

Keywords:
RNA structureRNA‐RNA interactionschemical probingnucleic acidsstructure determination

More Related Videos

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

32.2K
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

15.5K

Related Experiment Videos

Last Updated: Jan 22, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

5.1K
RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

32.2K
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

15.5K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Cellular RNA molecules possess intricate structures crucial for their functions.
  • Studying RNA structure within the native cellular environment is difficult due to limitations of in silico and in vitro methods.
  • The cellular environment significantly influences RNA structure, a factor often missing in traditional experimental setups.

Purpose of the Study:

  • To review advances in RNA structure probing techniques for studying RNA in its native cellular context.
  • To highlight novel chemical probes and adduct detection methods for RNA structure analysis.
  • To discuss the application of these methods for understanding the RNA structurome at single-molecule and single-cell levels.

Main Methods:

  • Development of cell-permeable chemical probes for RNA structure mapping.
  • Integration of RNA structure probing with second- and third-generation sequencing technologies.
  • Application of advanced adduct detection techniques for high-resolution RNA structure determination.

Main Results:

  • RNA structure probing enables capture of the RNA structural landscape in vivo.
  • Advanced sequencing and detection methods facilitate deep analysis of the RNA structurome.
  • These techniques allow examination of RNA structure at single-molecule, single-cell, and subcellular levels.

Conclusions:

  • RNA structure probing coupled with sequencing represents a powerful approach to study the functional RNA structurome.
  • Future directions include developing more innovative methodologies for unique biological questions.
  • This field offers profound insights into RNA biogenesis, regulation, and function within the cell.