Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cerebrospinal Fluid01:21

Cerebrospinal Fluid

5.5K
Cerebrospinal fluid (CSF) is a colorless liquid that flows around the brain and the spinal cord, playing a vital role in the protection, support, and overall function of the central nervous system (CNS). CSF production, circulation, and absorption are tightly regulated processes essential for the brain and spinal cord to function properly.
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain....
5.5K
The Fluid Mosaic Model01:34

The Fluid Mosaic Model

177.3K
The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.
177.3K
Multiple Allele Traits01:49

Multiple Allele Traits

38.0K
The Concept of Multiple Allelism
38.0K
Accelerating Fluids01:17

Accelerating Fluids

2.3K
When a fluid is in constant acceleration, the pressure and buoyant force equations are modified. Suppose a beaker is placed in an elevator accelerating upward with a constant acceleration, a. In the beaker, assume there is a thin cylinder of height h with an infinitesimal cross-sectional area, ΔS.
The motion of the liquid within this infinitesimal cylinder is considered to obtain the pressure difference. Three vertical forces act on this liquid:
2.3K
Fluid Pressure01:14

Fluid Pressure

1.2K
In mechanical engineering, fluid pressure plays a critical role in designing systems that utilize liquid flow, such as hydraulic systems, pumps, and valves. When designing these systems, engineers must ensure they can withstand the forces created by fluid pressure to avoid damage or failure.
According to Pascal's law, a fluid at rest will generate equal pressure in all directions. This pressure is measured as a force per unit area, and its magnitude depends on the fluid's specific...
1.2K
Characteristics of Fluids01:31

Characteristics of Fluids

986
Fluids differ from solids primarily in their molecular structure and stress response. Solids have tightly packed molecules with strong intermolecular forces, maintaining their shape and resisting deformation. In contrast, fluids have molecules spaced farther apart with weaker forces, allowing them to flow and deform easily.
Fluids, which include both liquids and gases, are substances that deform continuously under shearing stress. For example, water and oil are liquids with molecules that can...
986

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reversible & Microbubble Concentration-Dependent Permeabilization of an <i>In Vitro</i> Human Endothelial Barrier to Small Molecules Using Ultrasound: Implications for Neurodegenerative Diseases Therapy.

Molecular pharmaceutics·2026
Same author

Anchoring ALS Prognosis: Neurofilament Light Chain Outperforms Inflammatory, Metabolic, and CNS Barrier Biomarkers in the METABALS Cohort.

Molecular neurobiology·2026
Same author

Integrative analysis of circulating biomarkers of colorectal cancer combining mid-infrared spectroscopy and targeted metabolomics.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Functional changes in the gut microbiota are associated with the intestinal phenotype in A20 haploinsufficiency.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2026
Same author

Fecal tryptophan metabolism predicts the development of infant eczema: a prospective longitudinal study.

Gut microbes·2026
Same author

Saliva and plasma metabolome analysis during the five days before foaling in the mare.

PloS one·2026
Same journal

An Efficient TetR/TetO-Integrated Packaging System for Fowl Adenovirus 4 Vector Carrying Toxic Transgene.

Methods and protocols·2026
Same journal

Exploring Barriers and Facilitators to COVID-19 Vaccination Uptake Among Individuals with Mental Illness in the Australian Healthcare System: A Qualitative Study Protocol.

Methods and protocols·2026
Same journal

In Vitro Capacitation in Boar Sperm: Evaluation of Selected Detection Techniques.

Methods and protocols·2026
Same journal

Multiparametric Flow Cytometry Panel for Characterization of Mouse T Cell Differentiation and NK Cell Maturation Following Inflammatory Challenge.

Methods and protocols·2026
Same journal

Protocol for the Implementation of a Targeted Maternal and Newborn Service Delivery Bundle in Sierra Leone.

Methods and protocols·2026
Same journal

Contact Lens-Associated Ocular Surface and Corneal Disorders.

Methods and protocols·2026
See all related articles

Related Experiment Video

Updated: Jan 22, 2026

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
10:23

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Published on: December 4, 2017

9.4K

Decoding Cerebrospinal Fluid: Integrative Metabolomics Across Multiple Platforms.

Antoine Presset1,2, Sylvie Bodard1, Antoine Lefèvre1,3

  • 1Imaging Brain & Neuropsychiatry iBrain U1253, Université de Tours, INSERM, 37032 Tours, France.

Methods and Protocols
|January 21, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a standardized method for collecting cerebrospinal fluid (CSF) and an integrated multiplatform metabolomic workflow. This approach enhances the comprehensive analysis of the CSF metabolome for biomarker discovery and understanding neurochemical changes.

Keywords:
1H-NMR spectroscopyHPLC-MSbiochemical pathwaysbiomarkerscerebrospinal fluid (CSF)metabolomics

More Related Videos

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
06:40

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Published on: March 19, 2018

66.3K
Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid
06:21

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

Published on: April 7, 2023

2.0K

Related Experiment Videos

Last Updated: Jan 22, 2026

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
10:23

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Published on: December 4, 2017

9.4K
An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
06:40

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Published on: March 19, 2018

66.3K
Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid
06:21

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

Published on: April 7, 2023

2.0K

Area of Science:

  • Neuroscience
  • Metabolomics
  • Biochemistry

Background:

  • Cerebrospinal fluid (CSF) is crucial for central nervous system (CNS) health, reflecting its physiological and pathological states.
  • CSF regulates ionic balance, molecular transport, and waste clearance, supporting brain function.
  • Current methods for CSF metabolomic analysis may lack comprehensive coverage and robustness.

Purpose of the Study:

  • To present a standardized protocol for CSF collection.
  • To introduce an integrative multiplatform metabolomic workflow for CSF analysis.
  • To enhance the comprehensive and reliable characterization of the CSF metabolome for biomarker discovery.

Main Methods:

  • Standardized cerebrospinal fluid (CSF) collection protocol.
  • Integrative multiplatform metabolomic workflow.
  • Proton nuclear magnetic resonance spectroscopy (¹H-NMRS).
  • High-performance liquid chromatography coupled to mass spectrometry (HPLC-MS).

Main Results:

  • Integration of ¹H-NMRS and HPLC-MS enhances metabolite coverage.
  • Improved analytical robustness for CSF metabolome characterization.
  • Facilitates comprehensive and reliable analysis of CSF biomarkers.

Conclusions:

  • The presented workflow enables a more thorough understanding of the CSF metabolome.
  • Supports the discovery of novel biomarkers for CNS conditions.
  • Advances knowledge of neurochemical alterations in the central nervous system.