Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

10.4K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.4K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

8.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.3K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

7.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.2K
Notch Signaling Pathway03:14

Notch Signaling Pathway

6.5K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.5K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

9.9K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
9.9K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

8.8K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harnessing Chlorella vulgaris-derived extracellular vesicles for potentiated cancer immunotherapy.

Biomaterials·2026
Same author

Magnetic resonance imaging interpretation of temporomandibular joint disc position in mandibular condylar fractures: A fossa-based reference approach.

Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery·2026
Same author

Factors influencing adolescents' participation in school recess activities in urban and rural schools: A qualitative study.

Journal of exercise science and fitness·2026
Same author

Infill Pattern-Dependent Mechanical Properties and In Vitro Release Behavior of FDM 3D-Printed Resveratrol Amorphous Solid Dispersion Matrix Tablets.

Polymers·2026
Same author

Efficacy and safety of immune checkpoint inhibitors combined with tyrosine kinase inhibitors in patients with metastatic renal cell carcinoma: a risk-stratified systematic review and meta-analysis.

Frontiers in immunology·2026
Same author

Study on Regional Evolution Characteristics of Coal Spontaneous Combustion and Gas Coupling Disaster in Composite Goaf with No-Pillar Mining.

ACS omega·2026

Related Experiment Video

Updated: Jan 23, 2026

Obtaining Specimens with Slowed, Accelerated and Reversed Aging in the Honey Bee Model
10:58

Obtaining Specimens with Slowed, Accelerated and Reversed Aging in the Honey Bee Model

Published on: August 29, 2013

11.7K

Oral Delivery of R-spondin1-Loaded Small Extracellular Vesicles Activates WNT Signalling Pathway to Accelerate

Lingyan Yang1,2,3, Xu Wang1, Xiyang Wei1

  • 1Guangzhou National Laboratory, Guangzhou, Guangdong Province, China.

Journal of Extracellular Vesicles
|January 22, 2026
PubMed
Summary

Oral delivery of R-spondin1 (RSPO1) protein encapsulated in small extracellular vesicles (sEV) effectively rejuvenates the intestine. This therapeutic approach promotes intestinal stem cell (ISC) proliferation and accelerates tissue repair in injury and aging models.

Keywords:
R‐spondin1WNT signallingageing reversalextracellular vesiclesintestinal regeneration

More Related Videos

Author Spotlight: Deciphering Coagulation Disorders in Traumatic Brain Injury Patients
04:56

Author Spotlight: Deciphering Coagulation Disorders in Traumatic Brain Injury Patients

Published on: August 4, 2023

1.2K
Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

1.9K

Related Experiment Videos

Last Updated: Jan 23, 2026

Obtaining Specimens with Slowed, Accelerated and Reversed Aging in the Honey Bee Model
10:58

Obtaining Specimens with Slowed, Accelerated and Reversed Aging in the Honey Bee Model

Published on: August 29, 2013

11.7K
Author Spotlight: Deciphering Coagulation Disorders in Traumatic Brain Injury Patients
04:56

Author Spotlight: Deciphering Coagulation Disorders in Traumatic Brain Injury Patients

Published on: August 4, 2023

1.2K
Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

1.9K

Area of Science:

  • Gastroenterology
  • Regenerative Medicine
  • Biotechnology

Background:

  • The intestine is vital for metabolism and immunity, but its function declines with age and injury.
  • Activating the WNT/β-catenin pathway promotes intestinal stem cell (ISC) regeneration, crucial for repair.
  • Oral delivery of WNT agonists to the gut is a significant challenge.

Purpose of the Study:

  • To develop an orally deliverable therapeutic for intestinal regeneration.
  • To investigate the efficacy of WNT/β-catenin signalling agonists delivered via small extracellular vesicles (sEVs).

Main Methods:

  • Engineered small extracellular vesicles (sEVs) to display active R-spondin1 (RSPO1) protein on their surface.
  • Administered RSPO1-loaded sEVs (evRSPO1) orally in radiation-induced intestinal injury and aged mouse models.
  • Assessed WNT/β-catenin pathway activation, ISC proliferation, and tissue repair.

Main Results:

  • evRSPO1 successfully induced WNT/β-catenin signalling and ISC proliferation in vitro and in vivo.
  • Oral evRSPO1 accelerated tissue repair in a radiation-induced intestinal injury model.
  • evRSPO1 treatment reversed intestinal senescence and promoted ISC proliferation in aged mice.

Conclusions:

  • evRSPO1 is a potent, orally deliverable therapeutic for intestinal repair and rejuvenation.
  • This approach overcomes delivery barriers, activating ISCs for efficient tissue regeneration.
  • evRSPO1 represents a promising first-in-class therapy for age-related and injury-induced intestinal decline.