Related Experiment Video
Updated: Jan 24, 2026
08:27
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
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A FRET-based ratiometric fluorescent probe for selective hydrazine detection, live imaging, and portable sensing
Shuqi Tang1, Hongda Wu1, Yifeng Han1
1Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|January 22, 2026
Summary
We developed new fluorescent probes for sensitive and selective detection of toxic hydrazine. The best probe, RC-n-Bu, enables real-time monitoring in cells, zebrafish, and via test strips.
Area of Science:
- Analytical Chemistry
- Biomedical Engineering
- Environmental Science
Background:
- Hydrazine (N2H4) is a toxic industrial chemical.
- Sensitive and selective detection methods for hydrazine are crucial.
Purpose of the Study:
- To develop novel ratiometric fluorescent probes for hydrazine detection.
- To evaluate probe performance in biological and environmental samples.
Main Methods:
- Fabrication of FRET-based fluorescent probes integrating coumarin and rhodol.
- Assessment of probe performance including selectivity, sensitivity, and kinetics.
- Application of probes in live-cell imaging, zebrafish studies, and test strip development.
Main Results:
- Developed four ratiometric fluorescent probes (RC-Ac, RC-Pr, RC-n-Bu, RC-i-Bu).
- RC-n-Bu demonstrated optimal performance with high selectivity and a second-order rate constant of 49.4 M⁻¹·min⁻¹.
- Successful application in live-cell and zebrafish imaging, and visual detection using test strips with smartphone analysis.
Conclusions:
- The developed probes offer a versatile platform for hydrazine monitoring.
- RC-n-Bu provides precise quantification and excellent selectivity for hydrazine.
- The test strip format enables convenient on-site detection of hydrazine.
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