Influence of HIF1A-AS2 and miR-590-3p on trophoblast function and inflammation control in HTR-8/SVneo trophoblast...

Hypoxia-inducible factor 1 alpha antisense RNA 2 (HIF1A-AS2) and microRNA-590-3p (miR-590-3p) have been linked to inflammation and preeclampsia. In HTR-8/SVneo cells, HIF1A-AS2 was overexpressed and miR-590-3p mimic was introduced, and proliferation, migration/invasion and apoptosis were assessed by proliferation assays, wound-healing and Matrigel invasion assays, and flow cytometry, while molecular alterations were analyzed by quantitative reverse transcription polymerase chain reaction, western blotting, and immunofluorescence. HIF1A-AS2 overexpression enhanced proliferation and motility and reduced apoptosis, increased insulin-like growth factor 2 messenger RNA-binding protein 1 (IGF2BP1), and decreased NLR family pyrin domain containing 3 (NLRP3), gasdermin D (GSDMD) and cleaved caspase-1. Conversely, miR-590-3p suppressed IGF2BP1 and partially reversed HIF1A-AS2-associated phenotypes. Together, these data defined a HIF1A-AS2-miR-590-3p-IGF2BP1 axis linking trophoblast behaviour to inflammatory effector signalling.