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Updated: Jan 24, 2026

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
Published on: January 2, 2020
Ce-Doped Lignin-Based Nanozyme with Heat-Activated Hydrolase Activity
Xin Liu1,2, Lijun Li1,3, Xue Zhang3
1Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
Abstract:
Nanozymes are powerful alternatives to natural enzymes, yet the sustainable design of hydrolase nanozymes using renewable ligands remains underexplored. Lignin, as an abundant aromatic biopolymer, offers a promising and green ligand for constructing sustainable nanozymes. In this work, a Ce-doped lignin-based hydrolase nanozyme (Ce-AL) is synthesized using Ce ions coordinated with aminated industrial lignin. The resulting Ce-Nx centered Ce-AL enable the hydrolysis of phosphate esters and even stable protein amide bonds. Notably, the hydrolase-like activity of Ce-AL at 100°C is 2.03 times that at 40°C, attributable to the thermally optimized Ce-Nx sites within the protective lignin scaffold. The Ce-AL effectively combats stubborn biofilms formed by Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria by selectively hydrolyzing key proteinaceous and nucleotide components of the extracellular polymeric substances and bacterial cell walls. This work highlights lignin as a renewable platform for designing advanced, stimuli-responsive nanozymes.
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