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Updated: Jan 24, 2026
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Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
Published on: January 2, 2020
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Ce-Doped Lignin-Based Nanozyme with Heat-Activated Hydrolase Activity.
Xin Liu1,2, Lijun Li1,3, Xue Zhang3
1Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|January 23, 2026
Summary
This study introduces a novel cerium-doped lignin-based nanozyme (Ce-AL) that mimics hydrolase enzymes. This sustainable nanozyme effectively breaks down stubborn biofilms by targeting essential bacterial components.
Area of Science:
- Materials Science
- Biotechnology
- Nanotechnology
Background:
- Natural enzymes face limitations in stability and cost.
- Developing sustainable nanozymes with renewable ligands is crucial.
- Lignin, an abundant biopolymer, presents a promising green ligand for nanozyme construction.
Purpose of the Study:
- To synthesize and characterize a novel cerium-doped lignin-based hydrolase nanozyme (Ce-AL).
- To investigate the hydrolase-like activity and stability of Ce-AL.
- To evaluate the efficacy of Ce-AL against bacterial biofilms.
Main Methods:
- Synthesis of Ce-AL via coordination of Ce ions with aminated industrial lignin.
- Assay of hydrolase-like activity on phosphate esters and protein amide bonds.
- Evaluation of biofilm inhibition against *Escherichia coli* and *Staphylococcus aureus*.
Main Results:
- Ce-AL exhibits significant hydrolase activity, cleaving phosphate esters and protein amide bonds.
- Ce-AL demonstrates enhanced activity at higher temperatures (100°C vs. 40°C) due to thermally optimized Ce-Nx sites.
- Ce-AL effectively degrades Gram-negative and Gram-positive bacterial biofilms by targeting extracellular polymeric substances.
Conclusions:
- Lignin serves as a viable renewable platform for designing advanced, stimuli-responsive nanozymes.
- Ce-AL offers a sustainable and effective approach for combating bacterial biofilms.
- The developed nanozyme showcases potential for applications requiring robust hydrolase activity.
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