Related Experiment Video
Updated: Jan 25, 2026

Isolation of Murine Coronary Vascular Smooth Muscle Cells
Published on: May 30, 2016
Protocol for differentiation of vascular smooth muscle cells from human iPSCs and their application in
Laura Priesmeier1, Malte Tiburcy2, Laura Cecilia Zelarayán3
1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, 37075 Lower Saxony, Germany; German Center for Cardiovascular Research (DZHK), Lower Saxony, Germany; Clinic for Cardiology and Pneumology, University Medical Center Goettingen, 37075 Lower Saxony, Germany.
Abstract:
Directed differentiation of human induced pluripotent stem cells (hiPSCs) holds major promise for the development of disease models, drug screening platforms, and regenerative medicine. Here, we provide a step-by-step highly reproducible protocol for differentiating vascular smooth muscle cells (vSMCs) from hiPSCs, including hiPSC culture, hiPSC differentiation, and vSMC passaging under chemically defined conditions. We also detail molecular and functional analysis procedures for hiPSC-derived contractile vSMCs along with endogenous transcriptional activation modulation ready for any downstream application.
Related Concept Videos
Functions of Smooth Muscles
Function of visceral smooth muscles
Visceral smooth muscle is found in the walls of all hollow organs, except the heart, and is a key player in the involuntary movements that drive the functioning of these internal organs. This tissue is arranged in...
Smooth Muscle Contraction
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
Structure and Organization of Smooth Muscles
Structure of smooth muscle cell
Smooth muscle cells are spindle-shaped with tapering ends and a...
Master Transcription Regulators
Cell Specific Gene Expression
Gene Regulation During Sporulation

