Related Experiment Video
Updated: Jan 25, 2026

Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
Alkyl glucamine-based mixed-mode stationary phase for reversed-phase and hydrophilic interaction liquid
Shuo Wang1, Yiming Gu2, Jin Zhou2
1Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Abstract:
In modern liquid chromatography, versatile mixed-mode stationary phases have emerged as a practical choice for complex separations. Glucamine is promising in this field due to its distinctive amino-substituted glucose structure. This work presents a novel alkyl glucamine-based stationary phase (SIL-GNOGA) prepared via an epoxide-amine ring-opening reaction and immobilized onto silica surfaces. The morphology and composition of the stationary phase were characterized by multiple analytical techniques. Its retention mechanism was systematically investigated using analytes with diverse properties under varied chromatographic conditions. Furthermore, the separation performance of SIL-GNOGA was assessed and compared against its alkylamine- and glucamine-based counterparts. The stationary phase demonstrated excellent versatility, enabling efficient separation of hydrophilic, hydrophobic, as well as amphiphilic analytes in hydrophilic interaction or reversed-phase mode, depending on their molecular characteristics. Notably, complex mixtures were successfully resolved in both modes through the synergistic effects of hydrophilic, hydrophobic, hydrogen-bonding, and electrostatic interactions. This mixed-mode capability highlights SIL-GNOGA's broad selectivity and potential across diverse chromatographic applications.
More Related Videos
08:06Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
Published on: October 25, 2019
07:57Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
Published on: December 3, 2009
Related Concept Videos
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Phase Diagrams
Phase Transitions
Phase Transitions: Sublimation and Deposition
Phase Transitions: Melting and Freezing
Inductance: Single-Phase And Three-Phase Line
Single-Phase Two-Wire Line:
A single-phase line consists of two solid cylindrical conductors, denoted as x and y. Each conductor carries phasor currents ix and iy, respectively. Given that the sum of these currents is...