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Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Related Experiment Video

Updated: Jan 25, 2026

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

Large-scale spatial profiling of the tumor microenvironment.

Aaron Kilgallon¹, Rafael Tubelleza¹, Arutha Kulasinghe¹

¹Queensland Spatial Biology Centre, Wesley Research Institute, Brisbane, QLD, Australia; Frazer Institute, Faculty of Health, Medicine and Behavioural Sciences, University of Queensland, Brisbane, QLD, Australia.

|January 23, 2026

Summary

Researchers analyzed the tumor immune microenvironment in over 14,000 patients using multiplex immunofluorescence proteomics. This expansive analysis aids in cancer discovery and patient stratification.

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Last Updated: Jan 25, 2026

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

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Published on: September 13, 2022

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Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

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Published on: February 7, 2025

Area of Science:

Spatial biology
Proteomics
Cancer research

Background:

Multiplex immunofluorescence proteomics is key for understanding tissue architecture and cell states.
The tumor immune microenvironment significantly impacts cancer progression and treatment response.

Purpose of the Study:

To perform a large-scale population analysis of the tumor immune microenvironment.
To leverage spatial biology techniques for clinical discovery and patient stratification.

Main Methods:

Development of GigaTIME, a novel analytical platform.
Application of multiplex immunofluorescence proteomics to over 14,000 patient samples.
Analysis across 24 distinct cancer types.

Main Results:

Characterization of the tumor immune microenvironment at an unprecedented scale.
Identification of patterns and biomarkers for clinical discovery.
Demonstration of GigaTIME's utility in patient stratification.

Conclusions:

GigaTIME enables robust analysis of the tumor immune microenvironment.
Large-scale spatial proteomics facilitates significant clinical insights in oncology.
This approach holds promise for advancing precision oncology.