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Updated: Jan 25, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
Published on: April 29, 2010
Automated mapping of DNA replication fork progression in human cells with ForkML
Victoria Rojat1, Diletta Ciardo1, Alan Tourancheau1
1IBENS, Département de biologie, École Normale Supérieure, Université PSL, CNRS, INSERM, Paris, France.
Abstract:
Current approaches to mapping fork progression in the human genome suffer from drastically low throughput. Here, we introduce ForkML, a nanopore sequencing-based method automatically positioning thousands of individual fork velocities by tracking BrdU incorporation into replicating DNA after double pulse-labelling of asynchronous cells. ForkML recovers known human fork speed, accurately detects replication stress, and, crucially, connects replication dynamics to genomic and chromatin contexts, exposing fork slowdown in early-replicating transcribed regions.
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