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Chitooligosaccharide-modified ferritin core-shell carrier enhances rutin stability and slow release performance
Xiaoyue Liang1, Zhikun Xing1, Chen Chen1
1Key Laboratory of Characteristics Agricultural Product Processing and Quality Control (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China; Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China; School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China.
None:
This study developed a novel core-shell delivery system for the polyphenol rutin, utilizing the pH-responsive properties of Northern pike liver ferritin (NPLF). It encapsulates rutin therein, yielding a core-shell composite nanoparticle of Chitooligosaccharide-Apoferritin-Rutin (COS-Apo-Rutin) synthesized via electrostatic adsorption of COS. COS-Apo-Rutin particles exhibit a sphere-like morphology with nano-scale diameters, as observed by dynamic light scattering and transmission electron microscopy (TEM). The system demonstrated an 18.4% encapsulation efficiency, improved thermal stability (+4.38 °C). DPPH radical scavenging experiments showed a gradient decrease in antioxidant activity: Free Rutin > Apo-Rutin > COS-Apo-Rutin. In vitro simulated digestion analysis indicated that COS-Apo-Rutin exhibits more resistance to pepsin and trypsin digestion compared to ferritin alone. COS-Apo-Rutin demonstrated a slow release of Rutin within the simulated gastrointestinal tract. This core-shell system enhances the stability and delivery performance of Rutin, enabling improved utilisation of fish by-products and targeted nutrient delivery.
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