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Chitooligosaccharide-modified ferritin core-shell carrier enhances rutin stability and slow release performance
Xiaoyue Liang1, Zhikun Xing1, Chen Chen1
1Key Laboratory of Characteristics Agricultural Product Processing and Quality Control (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China; Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China; School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China.
Researchers created a novel nanoparticle delivery system using fish ferritin to enhance rutin stability and absorption. This Chitooligosaccharide-Apoferritin-Rutin (COS-Apo-Rutin) system improves polyphenol delivery for better health benefits.
Area of Science:
- Biomaterials Science
- Nutritional Science
- Nanotechnology
Background:
- Polyphenols like rutin offer health benefits but suffer from poor stability and bioavailability.
- Ferritin, a natural protein shell, shows potential for encapsulating bioactive compounds.
- Northern pike liver ferritin (NPLF) presents a pH-responsive platform for novel delivery systems.
Purpose of the Study:
- To develop a core-shell nanoparticle system for improved rutin delivery.
- To utilize NPLF and Chitooligosaccharide (COS) for enhanced rutin stability and controlled release.
- To evaluate the physicochemical properties, antioxidant activity, and in vitro digestion resistance of the novel system.
Main Methods:
- Synthesis of core-shell nanoparticles (COS-Apo-Rutin) via electrostatic adsorption.
- Characterization of particle morphology and size using Dynamic Light Scattering and Transmission Electron Microscopy (TEM).
- Assessment of encapsulation efficiency, thermal stability, antioxidant activity (DPPH assay), and in vitro simulated digestion.
Main Results:
- Successful synthesis of sphere-like COS-Apo-Rutin nanoparticles with nano-scale diameters.
- Achieved 18.4% encapsulation efficiency and improved thermal stability (+4.38 °C).
- Demonstrated reduced antioxidant activity in the order: Free Rutin > Apo-Rutin > COS-Apo-Rutin, with enhanced resistance to pepsin and trypsin digestion.
Conclusions:
- The COS-Apo-Rutin core-shell system effectively enhances rutin stability and delivery performance.
- This nanoparticle system shows potential for improved utilization of fish by-products and targeted nutrient delivery.
- The pH-responsive NPLF-based system offers a promising strategy for delivering bioactive compounds like rutin.
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