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Fluorescent indicators for visualizing dynamic contact between cells and between processes originating from a single

Takashi Kanadome1, Natsumi Hoshino2, Susumu Jitsuki3

  • 1Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-0012, Japan; Laboratory of Neuronal Cell Biology, National Institute for Basic Biology, Okazaki, Aichi 444-8787, Japan; Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan; Department of Basic Biology, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi 444-8787, Japan; Research Core, Mie University, Tsu, Mie 514-8507, Japan; SANKEN, The University of Osaka, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.

Cell Reports Methods
|January 24, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed Gachapin and Gachapin-C, novel fluorescent indicators for visualizing dynamic cell-cell contacts in living cells. These tools aid in studying cell communication and biological processes, offering new insights into cellular interactions.

Keywords:
CP: imagingbioimagingcell-cell contactddGFPfluorescent indicatorneuronal self-recognition

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Area of Science:

  • Cell Biology
  • Microscopy
  • Biophysics

Background:

  • Cell-cell contacts are crucial for multicellular organism function.
  • Visualizing dynamic cell interactions in living cells is essential for understanding biological processes.
  • Existing tools for visualizing cell contacts have limitations.

Purpose of the Study:

  • To develop novel fluorescent indicators for visualizing dynamic cell-cell contacts.
  • To enable simultaneous monitoring of contact dynamics, cytoskeletal assembly, and intracellular signaling.
  • To provide tools for studying cell-cell contact-mediated processes in living cells.

Main Methods:

  • Development of two fluorescent indicators: Gachapin and Gachapin-C.
  • Utilizing Gachapin for visualizing both static and dynamic cell contacts.
  • Employing multiplexed imaging with Gachapin and spectrally distinct indicators.
  • Using Gachapin-C for single-component contact visualization.

Main Results:

  • Gachapin successfully visualizes static and dynamic cell-cell contacts.
  • Multiplexed imaging with Gachapin allows simultaneous observation of contact dynamics, cytoskeletal assembly, and signaling.
  • Gachapin-C simplifies contact visualization, enabling monitoring of contacts within a single cell.
  • Formation and disruption of neuronal process contacts can be visualized.

Conclusions:

  • Gachapin and Gachapin-C are valuable tools for studying dynamic cell-cell contacts.
  • These indicators provide deeper insights into cell-cell contact-mediated processes.
  • The developed tools facilitate research in cell communication and fundamental biological processes.