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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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ATP Driven Pumps I: An Overview01:27

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ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
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ATP Driven Pumps II: P-type Pumps01:34

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The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
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Xylem and Transpiration-driven Transport of Resources02:03

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The xylem of vascular plants distributes water and dissolved minerals that are taken up by the roots to the rest of the plant. The cells that transport xylem sap are dead upon maturity, and the movement of xylem sap is a passive process.
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ATP Driven Pumps III: V-type Pumps01:30

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V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
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Cancer02:18

Cancer

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Cancers arise due to mutations in genes involved in the regulation of cell division, which leads to unrestricted cell proliferation. Modern science and medicine have made great strides in the understanding and treatment of cancer, including eradicating cancer in some patients. However, there is still no cure for cancer. This is largely due to the fact that cancer is a large group of many diseases.
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Orthotopic Mouse Model of Colorectal Cancer
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Deciphering Stiffness-Driven Changes in Colorectal Cancer by Proteomics.

Charlotte Cresens1, Ana Montero-Calle2, Guillermo Solís-Fernández3

  • 1Molecular Imaging and Photonics Division, Chemistry Department, Faculty of Sciences, KU Leuven, Heverlee, Belgium.

Molecular & Cellular Proteomics : MCP
|January 25, 2026
PubMed
Summary
This summary is machine-generated.

Tumor stiffening significantly alters colorectal cancer cell secretomes, enhancing migration and angiogenesis. This highlights matrix stiffness

Keywords:
colorectal cancerfunctional assaysintracellular and secreted proteomeproteomicssecretometumor stiffness

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Area of Science:

  • Biomedical Engineering
  • Cancer Biology
  • Proteomics

Background:

  • Tumor stiffening is a critical factor in cancer progression and metastasis.
  • The mechanical properties of the tumor microenvironment influence cancer cell behavior.
  • Limited research exists on the impact of tumor stiffness on protein expression.

Purpose of the Study:

  • To investigate the effect of matrix stiffness on protein dysregulation in colorectal cancer.
  • To identify specific protein changes in response to altered matrix stiffness.
  • To understand how these protein changes influence cancer progression.

Main Methods:

  • In-depth proteomics analysis of colorectal cancer cells.
  • Comparison of protein expression under varying matrix stiffness conditions.
  • Functional assays to assess cell migration, angiogenesis, and matrix remodeling.

Main Results:

  • Matrix stiffness significantly altered the expression of secreted proteins (secretome).
  • Intracellular protein levels remained largely unaffected by changes in matrix stiffness.
  • Stiffness-induced secretome changes promoted cell migration, angiogenesis, and matrix remodeling.

Conclusions:

  • Matrix stiffness plays a critical role in driving colorectal cancer progression via secretome alterations.
  • Changes in the secretome contribute to a more aggressive cancer phenotype.
  • Findings offer insights for developing novel biomechanical cancer therapies.