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Updated: Jan 27, 2026

Author Spotlight: Advancing Intestinal Bacteria Cultivation for Poultry
Published on: May 10, 2024
Isolation of anaerobic bacteria from bovine mastitis cases in Mombetsu district, Japan
Koji Nishi1,2, Noriko Imaizumi2, Ayako Eguchi-Nishi2,3
1Mombetsu Veterinary Clinic, Hokkaido Agricultural Mutual Aid Association, Hokkaido, Japan.
Abstract:
Bovine mastitis is an inflammatory disease of the udder caused by pathogen infection. In case of aerobic cultivation, 30% of milk samples have nonbacterial; this may relate to anaerobic bacteria, although this remains unclear. This study analyzed 173 mastitis milk samples collected via aerobic and anaerobic cultivation. The detection rate by anaerobic culture was significantly higher (P<0.01; 136 samples: 78.6%) than that by aerobic culture (125 samples: 72.3%). Furthermore, the detection rates of Streptococcus dysgalactiae, Streptococcus uberis, and Trueperella pyogenes were higher in anaerobic than aerobic culture. Clostridium perfringens was detected one sample (0.61%) and Fusobacterium necrophorum in two samples (1.22%) were detected. Overall, these findings indicated the value of anaerobic culture for detecting the causative bacteria of mastitis.
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