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Updated: Jan 27, 2026
05:49
Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
3.5K
Engineering of antimicrobial peptides by surface display technologies
Sugra Yuceer1, Husniye Tansel Yalcin2, Sibel Kalyoncu3
1Izmir Biomedicine and Genome Center, Izmir, Turkiye; Department of Biology, Ege University, Izmir, Turkiye.
Advances in Protein Chemistry and Structural Biology
|January 25, 2026
Summary
Antimicrobial peptides (AMPs) are natural defense molecules effective against drug-resistant bacteria. Protein engineering, particularly surface display, enhances AMPs for improved therapeutic applications against challenging pathogens.
Area of Science:
- Microbiology and Immunology
- Biotechnology and Protein Engineering
Background:
- Intensive antibiotic use has led to the rise of antibiotic-resistant pathogens, posing a significant threat to human health.
- Antimicrobial peptides (AMPs) represent a primitive defense mechanism in living organisms, offering a potential alternative to conventional antibiotics.
Purpose of the Study:
- To review the properties of antimicrobial peptides (AMPs) and their potential as therapeutic agents.
- To explore the application of protein engineering, specifically surface display technology, for developing improved AMPs.
Main Methods:
- Review of existing literature on antimicrobial peptides, their mechanisms of action, and therapeutic potential.
- Discussion of protein engineering strategies, focusing on surface display for high-throughput modification and screening of AMPs.
Main Results:
- AMPs exhibit broad-spectrum activity and unique membrane-active mechanisms that reduce resistance development.
- Challenges such as limited activity, host toxicity, and poor stability hinder the clinical application of natural AMPs.
Conclusions:
- AMPs hold significant therapeutic promise for combating antibiotic-resistant pathogens.
- Protein engineering, especially surface display, is a viable strategy to overcome AMP limitations and develop next-generation therapeutics.
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