Characterization of a Bioprinted Anticancer Cell Therapy System Generated with Continuous Liquid Interface...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Cancer Therapy

Background:

• Anticancer cell therapies show promise but face delivery challenges.
• 3D bioprinting (3DBP) offers a solution for creating cell delivery devices.
• Continuous liquid interface production (CLIP) is a 3DBP technology with potential for cell therapy.

Purpose of the Study:

• To investigate the feasibility of CLIP for 3D bioprinting cell-laden scaffolds.
• To evaluate the efficacy of CLIP-bioprinted cell therapies in vitro and in vivo.
• To establish CLIP as a platform for advanced cell therapy delivery devices.

Main Methods:

• Utilized CLIP 3D bioprinting with a gelatin methacrylate resin.
• Incorporated drug-secreting fibroblasts as a model anticancer therapy.
• Assessed cell viability, in vitro efficacy, and in vivo survival in a glioblastoma mouse model.

Main Results:

• CLIP enabled rapid and consistent production of cell-laden scaffolds.
• Printed cells maintained viability and anticancer efficacy post-printing.
• Bioprinted cells exhibited improved in vivo survival compared to direct injection, correlating with better outcomes.

Conclusions:

• CLIP is a viable technology for 3D bioprinting of cell therapy delivery devices.
• CLIP-bioprinted cell therapies demonstrate enhanced therapeutic potential against glioblastoma.
• This study provides a foundation for developing sophisticated cell therapy delivery systems using CLIP.