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Updated: Jan 28, 2026

Wholemount In Situ Hybridization for Astyanax Embryos
Published on: March 2, 2019
Arachniodes × tohtomiensis hyb. nov. (Dryopteridaceae) from Japan
Kiyotaka Hori1, Daiki Takahashi2, Ying-Hong He3
1The Kochi Prefectural Makino Botanical Garden, 4200-6 Godaisan, Kochi, Kochi 781-8125, Japan The Kochi Prefectural Makino Botanical Garden Kochi Japan.
Abstract:
This study aims to characterize a hybrid of Arachniodes (Dryopteridaceae), A. × tohtomiensis, for which molecular evidence of its origin and detailed morphological descriptions are lacking. The genome composition of A. × tohtomiensis, clarified based on plastid DNA and MIG-seq analyses, indicates that it is a diploid hybrid of A. exilis and an undetected species. This origin was also supported by phylogenetic, STRUCTURE, principal component, and neighbor-net analyses. Based on these evidences, it is formally described as A. × tohtomiensis Shimura & Hori, hyb. nov. It is morphologically characterized by its yellowish-green laminae and erose-to-ciliate indusia.
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