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Updated: Jan 28, 2026

Extraction and Analysis of Taiwanese Green Propolis
Published on: January 7, 2019
Influence of Propolis Extract on Bleaching Effectiveness and Enamel Physical Properties
Julliana Andrade da Silva1, Renan Alves E Cavalheiro1, Daniela Moreira Dos Santos1
1Department of Restorative Dentistry, Piracicaba School of Dentistry (FOP), State University of Campinas (UNICAMP), Piracicaba, SP 13414-903, Brazil.
Propolis extract, when used after 35% hydrogen peroxide (HP) bleaching, did not negatively impact dental enamel. This natural agent maintained tooth whitening effectiveness and protected enamel surface roughness and microhardness.
Area of Science:
- Dental materials science
- Biomaterials research
- Oral health
Background:
- Tooth bleaching with high-concentration hydrogen peroxide (HP) can alter dental enamel properties.
- Investigating protective agents is crucial to mitigate potential adverse effects of bleaching.
Purpose of the Study:
- To evaluate the effects of propolis extract on dental enamel following 35% HP bleaching.
- To assess the impact of propolis on color change, surface roughness, and microhardness of bovine enamel.
Main Methods:
- Sixty bovine enamel specimens were divided into six groups, including controls and various combinations of HP bleaching, desensitizers, fluoride, and propolis extract.
- Evaluated color change (ΔE*, ΔE00, ΔWID), surface roughness (Ra), and microhardness (KHN) at baseline and 24 hours post-bleaching.
Main Results:
- All HP-treated groups exhibited significant color change compared to the control.
- While bleaching increased surface roughness, propolis and desensitizer treatments reduced this effect.
- The combination of HP, desensitizer, and propolis maintained enamel microhardness comparable to the control.
Conclusions:
- Propolis extract does not compromise the whitening efficacy of 35% HP.
- Propolis extract shows a protective effect on dental enamel, preventing significant negative impacts on surface roughness and microhardness.
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