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Updated: Jan 28, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
Published on: June 28, 2024
Au/Ir@Zn/Cu-MOF-based lateral flow immunoassay employing high-affinity monoclonal antibody for sulfamethoxazole
Linfang Lu1, Lihong Wang1, Kang Jiang1
1The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins, School of Animal Science, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Abstract:
Sulfamethoxazole (SMX) is a widely used sulfonamide antibiotic, and its residues in animal-derived foods pose significant health risks. In this study, a high-affinity monoclonal antibody (2.65 × 1010 L/mol) against SMX was developed and used to construct an Au/Ir@Zn/Cu-MOF-based lateral flow immunoassay (LFIA). The probe was thoroughly characterized with good stability. The LFIA achieved a visual detection limit of 2.5 μg/mL, with an IC50 of 565.84 ng/mL and a linear detection range of 6.8 ng/mL-47.06 μg/mL. No cross-reactivity to structural analogues or matrix interference was observed in real food samples. Recovery rates (97.92-108%) were consistent with LC-MS results, confirming the accuracy of the method. This LFIA provides a sensitive and reliable tool for rapid SMX screening in food products.
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