Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

88.8K
Overview
88.8K
The Extracellular Matrix01:29

The Extracellular Matrix

12.1K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
12.1K
Extracellular Matrix01:26

Extracellular Matrix

5.4K
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
5.4K
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

3.4K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.4K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.3K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.3K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

9.0K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocol for Novel Perioperative Optimization of Obese Osteoarthritic Patients pending Total Knee Replacement with glucagon-like peptide-1 receptor agonist (NPO-OOPS-TKR) : a pilot randomized controlled trial.

Bone & joint open·2026
Same author

In-situ Damascus-patterning enables tunable surface electric fields for bioactive titanium implants.

Bioactive materials·2026
Same author

APLP2 as a molecular link between immune regulation and bone metabolism in hepatocellular carcinoma: evidence from scRNA-seq and functional validation.

Cancer cell international·2026
Same author

Association of dietary patterns with all-cause and cause-specific mortality in a high cardiovascular risk population in China.

BMC cardiovascular disorders·2026
Same author

Meaning in life of older persons with end-stage kidney disease: a scoping review protocol.

JBI evidence synthesis·2026
Same author

<i>In situ</i> generation of dendritic cell vaccines within 3D-printed scaffolds.

National science review·2026

Related Experiment Video

Updated: Jan 28, 2026

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
10:54

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Published on: January 17, 2017

12.1K

A Tunable Coacervate Matrix Emulates Embryonic Extracellular Matrix (ECM) Biophysical Cues To Regulate Stem Cell

Xian Xie1,2, Xu Yan2, Rongjie Wu2

  • 1Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong 999077, P. R. China.

Journal of the American Chemical Society
|January 27, 2026
PubMed
Summary

Researchers developed a tunable gelatin coacervate matrix to mimic early uterine environments. This biomimetic platform supports embryonic stem cell growth, stemness, and differentiation for developmental modeling.

More Related Videos

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

5.8K
Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
08:56

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Published on: August 1, 2010

17.9K

Related Experiment Videos

Last Updated: Jan 28, 2026

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
10:54

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Published on: January 17, 2017

12.1K
Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

5.8K
Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
08:56

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Published on: August 1, 2010

17.9K

Area of Science:

  • Biomaterials Science
  • Developmental Biology
  • Stem Cell Biology

Background:

  • Early embryogenesis is guided by dynamic biophysical changes in the uterine environment.
  • Replicating these specific in vitro cues for embryonic development is difficult.

Purpose of the Study:

  • To create a biomimetic matrix that recapitulates the biophysical cues of pre- and peri-implantation stages.
  • To develop a platform for studying early embryonic development and stem cell behavior.

Main Methods:

  • Developed a gelatin-based coacervate matrix with phase-transition-mediated tunable mechanics.
  • Utilized liquid-liquid phase separation for ultradynamic coacervate structures.
  • Investigated matrix properties during coacervate-solution and coacervate-hydrogel transitions.

Main Results:

  • The coacervate matrix exhibited volume expansion supporting cell proliferation and preserved stemness via moderate cell-matrix interactions.
  • The matrix facilitated efficient harvesting of viable embryonic stem cell colonies.
  • Matrix stiffening promoted peri-implantation-like invasive behaviors, including enhanced cell adhesion and ECM-degrading enzyme secretion.

Conclusions:

  • The tunable coacervate matrix is a versatile platform for clonal growth, stemness maintenance, and lineage initiation.
  • This biomimetic approach offers new opportunities for developmental modeling and therapeutic applications.