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Updated: Jan 28, 2026

Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro
Published on: April 15, 2022
Disinfection Strategies for Implant-Related Prosthetic Materials: An In Vitro Evaluation of Citric Acid,
Eduardo Escaf-Robles1, Aritza Brizuela-Velasco2, Daniel Robles-Cantero2
1Department of Surgery and Medical-Surgical Specialities, Oviedo University, 33003 Oviedo, Spain.
Abstract:
Background/Objectives: There is evidence of possible contamination of prosthetic components originating from dental laboratories. The aim of this study is to investigate the disinfectant effect of citric acid and polyethylene glycol on implant-prosthetic materials in comparison with an untreated control and chlorhexidine. Methods: A total of 720 disks made of three different materials (titanium grade V, zirconia coated with feldspathic ceramic, and PMMA) contaminated with three bacteria (Staphylococcus aureus, Enterococcus faecalis, and porphyromonas gingivalis) were analyzed. Four treatment groups were tested: citric acid, polyethylene glycol, chlorhexidine and an untreated control group. Two assessment periods (3 and 21 days of incubation) were used, with bacterial metabolic activity measured using the resazurin reduction test and then analyzed by electron microscopy. Results: The results show that chlorhexidine has a superior inhibitory effect on all materials and bacterial strains in the short-term evaluation (3 days), while citric acid and polyethylene glycol showed higher efficacy after 21 days. Citric acid also exhibits differential effects when applied to grade V titanium. These differences were statistically significant at p < 0.05. Conclusions: There is evidence to recommend chlorhexidine for the disinfection of laboratory prosthetic components, but the enhanced effect of citric acid on grade V titanium and its long-term efficacy make it clinically promising candidate.
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