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Updated: Jan 28, 2026
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Advanced Glycation End-Products Sensitize Human Sensory-Like Neuron Cells to Capsaicin-Induced Calcium Influx
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Multimatrix Detection and Quantification of the Advanced Glycation End Products Precursor Fructoselysine via
Simona Fenizia1,2, Marcello Manfredi1,2,3, Valentina Antoniotti4
1Department of Translational Medicine (DIMET), University of Piemonte Orientale, I-28100 Novara, Italy.
Metabolites
|January 27, 2026
Summary
A new mass spectrometry method accurately quantifies fructoselysine (FL), an advanced glycation end product (AGE) intermediate. Elevated FL levels were observed in prediabetic individuals, suggesting its potential role in early disease detection.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Metabolomics
Background:
- Advanced glycation end products (AGEs) are implicated in aging and metabolic diseases.
- Fructoselysine (FL), an AGE intermediate, accumulation is linked to adverse health outcomes.
- No mass spectrometry method existed for FL quantification in human biological samples.
Purpose of the Study:
- To develop and validate a novel UHPLC-HRMS/MS method for FL quantification.
- To establish a sensitive and reliable assay for FL in diverse biological matrices.
- To investigate FL levels in relation to prediabetes.
Main Methods:
- Development of a UHPLC-HRMS/MS assay.
- Validation of the method for linearity, accuracy, precision, LOD, LOQ, and recovery.
- Application of the method to quantify FL in human plasma, feces, and urine.
Main Results:
- The method exhibited excellent analytical performance (LOD 0.02 µM, LOQ 0.06 µM, RSD <10%).
- FL was successfully quantified in plasma, feces, and urine of healthy individuals.
- Higher circulating FL levels were detected in prediabetic patients compared to controls.
Conclusions:
- A validated UHPLC-HRMS/MS method enables sensitive FL detection in biological samples.
- Elevated FL may serve as an early biomarker for prediabetic conditions.
- This assay aids in understanding molecular changes in aging-related diseases for improved early diagnosis.
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