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Discovery of Antimicrobial Oligoindoles from a Cold-Seep-Derived Halomonas Strain
Yunchen Yan1, Zhiting Li1, Hongcheng Li1
1Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Abstract:
Mining bioactive secondary metabolites from microorganisms originating from deep-sea cold seep holds significant potential for discovering novel drug lead compounds. In this study, three known indole derivatives (1-3) were isolated from cold-seep-derived Halomonas meridiana OUCLQ22-B7. Subsequently, two-new indole dimers, meribisindole A (4) and meribisindole B (5), with nine known metabolites (6-14) were obtained via indole precursor feeding strategy. The structure of these compounds was elucidated via a combination of spectroscopic methods and circular dichroism (CD) measurement. Antimicrobial assays revealed that compounds 4, 7 and 8 exhibited potent inhibitory activity against Fusarium oxysporum CICC 41029 with minimal inhibitory concentrations (MICs) of 0.39-12.5 μg/mL, and compound 11 showed significant growth inhibition against Staphylococcus aureus CCARM 3090 with MIC value at 0.098 μg/mL.
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