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Updated: Jan 28, 2026
05:17
An Improved Method for the Preparation of Type I Collagen From Skin
Published on: January 21, 2014
23.4K
Optimized Conditions for Extracting Native Type-I Collagen from Discarded Fish Skin Using Hydrochloric Acid to
S T Gonapinuwala1,2, J R Jones1, S Kirk1
1School of Food and Natural Sciences, Massey University, Palmerston North 4442, New Zealand.
Marine Drugs
|January 27, 2026
Summary
This study presents a faster, cheaper method for extracting fish skin collagen using hydrochloric acid, preserving its native structure. This new approach avoids lengthy processing and dialysis, making fish collagen extraction more efficient.
Area of Science:
- Biochemistry
- Materials Science
- Sustainable Chemistry
Background:
- Fish skin is a valuable, underutilized by-product rich in type I collagen.
- Traditional acetic acid extraction is lengthy (>72h) and requires complex dialysis.
- Acetic acid residues can negatively impact collagen structure.
Purpose of the Study:
- To develop a simple, cost-effective, and time-efficient method for extracting fish skin collagen.
- To investigate the use of hydrochloric acid for collagen extraction.
- To provide guidelines for applying the method across different fish species.
Main Methods:
- Extraction of collagen using 0.01 M hydrochloric acid at a 1:20 mass to volume ratio for 5 hours.
- Homogenization of the fish skin extract.
- Characterization of collagen structure and thermal stability using ATR-FTIR, circular dichroism, and differential scanning calorimetry.
Main Results:
- Successfully extracted fish skin collagen with a preserved native triple-helical structure.
- Confirmed thermal stability of the extracted collagen.
- Established optimal extraction parameters including pH limit (4), manageable viscosity, and minimal undissolved material.
Conclusions:
- Hydrochloric acid offers a superior alternative to acetic acid for fish skin collagen extraction.
- The developed method is significantly faster and simpler, reducing costs and processing time.
- The findings provide a scalable protocol for efficient collagen extraction from various fish species.
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