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Therapeutically Induced Modulation of Collagen I-to-III Ratio Three Weeks After Rabbit Achilles Tendon Full
Gabriella Meier Bürgisser1, Olivera Evrova1, Pietro Giovanoli1
1Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, 8091 Zurich, Switzerland.
Biology
|January 28, 2026
Summary
Platelet-derived growth factor-BB (PDGF-BB) therapy enhanced collagen maturation in rabbit Achilles tendons. This therapeutic approach increased the collagen I-to-III ratio in specific healing zones, indicating improved tendon repair.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Orthopedic Research
Background:
- Tendon healing involves sequential collagen expression, with collagen III preceding collagen I.
- The collagen I-to-III ratio is a key indicator of tendon healing status.
- Understanding how therapies affect this ratio across anatomical zones is crucial for effective tendon repair.
Purpose of the Study:
- To compare the impact of platelet-derived growth factor-BB (PDGF-BB) delivered via electrospun mesh on the collagen I-to-III ratio in healing rabbit Achilles tendons.
- To evaluate the effectiveness of PDGF-BB therapy across different anatomical zones of the healing tendon.
- To assess the correlation between dual-color Herovici staining and single immunohistochemistry labeling for collagen I and III.
Main Methods:
- Utilized a rabbit Achilles tendon full transection model.
- Administered PDGF-BB via controlled release from coaxially electrospun DegraPol® hollow-fiber mesh.
- Compared PDGF-BB treatment with a vehicle-only control.
- Analyzed collagen I-to-III ratios at 3 weeks post-operation using dual-color Herovici staining and single immunohistochemistry (IHC).
- Examined distinct zones: native tendon, reactive zone, hot zone, and scaffold zone.
Main Results:
- Herovici staining (HV) showed weak correlation with immunohistochemistry (IHC) for collagen I-to-III ratios.
- PDGF-BB therapy increased collagen I-to-III ratios in most zones (except scaffold pores) based on HV staining.
- IHC analysis did not reveal significant differences in collagen ratios between PDGF-BB and control groups.
- Higher collagen I-to-III ratios were observed distal to the implant, in the core of the healing tendon, not in immediate proximity.
Conclusions:
- PDGF-BB therapy, delivered via electrospun mesh, suggests enhanced collagen maturation in specific zones of the healing tendon.
- Herovici staining may offer a more nuanced assessment of collagen ratios compared to standard IHC in this context.
- The distribution of collagen maturation indicates a localized but potentially beneficial effect of PDGF-BB on tendon healing.
Keywords:
Achilles tendonHerovicicollagen Icollagen IIIfull transection modelimmunohistochemistrymature collagenrabbittenotomiesyoung collagenMore Related Videos
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