Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutically Induced Modulation of Collagen I-to-III Ratio Three Weeks After Rabbit Achilles Tendon Full Transection.

Biology·2026
Same author

Electrospun DegraPol Tube Delivering Stem Cell/Tenocyte Co-Culture-Derived Secretome to Transected Rabbit Achilles Tendon-In Vitro and In Vivo Evaluation.

International journal of molecular sciences·2025
Same author

Synergistic Effects of Insulin-like Growth Factor-1 and Platelet-Derived Growth Factor-BB in Tendon Healing.

International journal of molecular sciences·2025
Same author

Therapeutic Potential of Mesenchymal Stem Cell and Tenocyte Secretomes for Tendon Repair: Proteomic Profiling and Functional Characterization In Vitro and In Ovo.

International journal of molecular sciences·2025
Same author

Fabrication and Characterization of Electrospun DegraPol<sup>®</sup> Tubes Releasing TIMP-1 Protein to Modulate Tendon Healing.

Materials (Basel, Switzerland)·2025
Same author

Standardization to Characterize the Complexity of Vessel Network Using the Aortic Ring Model.

International journal of molecular sciences·2025
Same journal

Spatial Heterogeneity of Phytoplankton Taxa and Functional Groups Under Multidimensional Environmental Factors in Karst Urban Rivers.

Biology·2026
Same journal

Paleopathology of a Lower Miocene Carettochelyid Turtle from the Moghra Formation, Egypt.

Biology·2026
Same journal

Effects of Type I Diabetes Mellitus and Masticatory Loading on Mandibular Growth in Growing Rats: A Longitudinal CBCT Study.

Biology·2026
Same journal

Data-Limited Stock Status Assessment of Bonga Shad, <i>Ethmalosa fimbriata</i> (Bowdich, 1825) and Lesser African Threadfin, <i>Galeoides decadactylus</i> (Bloch, 1795) in the Central Gulf of Guinea.

Biology·2026
Same journal

Gonadogenesis in the Bearded Dragon (<i>Pogona vitticeps</i>, Agamidae): A Comprehensive Histological Analysis from Gonadal Ridge Formation to Testicular and Ovarian Development.

Biology·2026
Same journal

The Programmable Microbiome: Integrative AI and Multi-Omics Frameworks for Precision T2DM Management.

Biology·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2026

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting
11:37

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

Published on: June 18, 2018

Tuning Secretomes for Regenerative Medicine.

Johanna Buschmann1

  • 1Division of Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstrasse 14, 8091 Zurich, Switzerland.

Biology
|June 25, 2026
PubMed
Summary
This summary is machine-generated.

Tailoring cell secretomes enhances regenerative medicine. Modulating culture conditions like media, formats, duration, and stress optimizes therapeutic potential for advanced treatments.

Keywords:
cell-cell communicationextracellular vesiclesgrowth factorproteintrophic factorwound healing

More Related Videos

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
08:38

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Published on: March 19, 2013

Related Experiment Videos

Last Updated: Jun 26, 2026

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting
11:37

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

Published on: June 18, 2018

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
08:38

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Published on: March 19, 2013

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Cell Biology

Background:

  • Cell-derived secretomes are crucial for tissue repair and regeneration.
  • Optimizing secretome composition requires understanding cellular responses to culture conditions.

Purpose of the Study:

  • To review methods for systematically tailoring cell-derived secretomes for regenerative medicine applications.
  • To analyze how various cell culture parameters influence secretome profiles.

Main Methods:

  • Systematic review of literature on cell secretome engineering.
  • Evaluation of diverse cell sources (platelets, MSCs, etc.).
  • Analysis of critical culture parameters: media supplementation, 2D vs. 3D formats, culture duration, fluidics, and microenvironmental stress (hypoxia, oxidative stress).

Main Results:

  • Cell source selection significantly impacts secretome composition.
  • Defined media, supplements, and priming agents precisely modulate secretome profiles.
  • 3D spheroid cultures and dynamic systems can enhance therapeutic factor secretion compared to 2D monolayers and static conditions.
  • Microenvironmental stresses like hypoxia can alter the paracrine signaling of secretomes.

Conclusions:

  • Systematic manipulation of cell culture conditions offers a powerful strategy for engineering potent cell-derived secretomes.
  • Precise control over secretome fabrication is key to advancing their application in regenerative medicine.
  • Further research into optimizing culture parameters will unlock the full therapeutic potential of secretomes.