Related Experiment Video
Updated: Jan 29, 2026
06:36
Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
Published on: August 21, 2020
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Nonthermal Atmospheric Plasma Modulates Palatal Wound Healing in Rats: A Morphometric, Histopathologic and
Basak Kusakci Seker1, Hakan Ozdemir2, Suna Karadeniz Saygili3
1Dentarma Private Oral and Dental Health Clinic, 07000 Antalya, Türkiye.
Biomedicines
|January 28, 2026
Summary
Non-thermal atmospheric plasma (NTAP) significantly enhances oral mucosal healing by promoting cell proliferation and tissue repair. This study shows NTAP accelerates wound closure and improves vascularization in palatal wounds.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Oral Surgery
Background:
- Non-thermal atmospheric plasma (NTAP) shows potential for tissue regeneration by modulating cellular signaling.
- Oral mucosal healing mechanisms and molecular pathways influenced by NTAP require further investigation.
Purpose of the Study:
- To investigate the histological and immunohistochemical effects of NTAP on palatal wound healing in rats.
- To evaluate key biomarkers (VEGF-A, TGF-β, FGF-2, CD34, α-SMA, Ki67) in angiogenesis, proliferation, and extracellular matrix remodeling during NTAP-treated wound healing.
Main Methods:
- Sixty rats underwent palatal wound creation and were divided into Saline Control Group (SCG), Chlorhexidine Gluconate Group (CHG), and NTAP-Treated Group (NTAPG).
- Histological and immunohistochemical analyses were performed on postoperative days 7, 14, and 21 to assess vascularization, inflammation, collagen deposition, epithelialization, and biomarker expression.
Main Results:
- NTAP treatment significantly increased TGF-β levels, α-SMA-positive cells, FGF-2, VEGF-A, and CD34 expression, indicating enhanced proliferation and angiogenesis.
- NTAP reduced inflammatory cell infiltration and led to improved fibroblast proliferation, collagen deposition, vascularization, and re-epithelialization compared to controls.
- Histological assessments revealed accelerated wound healing in the NTAP group.
Conclusions:
- NTAP significantly promotes palatal wound healing through enhanced proliferative activity and growth factor expression.
- NTAP accelerates tissue repair, suggesting its potential as an effective therapeutic for oral mucosal wound healing.
- The study highlights NTAP's efficacy in improving key healing parameters in oral mucosa.
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