Related Experiment Video
Updated: Jan 29, 2026
07:35
Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
Published on: December 16, 2021
2.8K
Cell Surface Shaving-Based Proteomic Profiling of the Surfaceome in Pathogenic Microorganisms
Dorota Satala1, Katarzyna Kowalik1,2, Justyna Karkowska-Kuleta1
1Department of Comparative Biochemistry and Bioanalytics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Gronostajowa 7, 30-387 Krakow, Poland.
International Journal of Molecular Sciences
|January 28, 2026
Summary
Microbial surface proteins, the surfaceome, are crucial for host-pathogen interactions. The cell surface shaving technique reveals these proteins and their roles in infection development.
Area of Science:
- Microbiology
- Immunology
- Biochemistry
Background:
- The microbial cell wall is vital for cell integrity, growth, and environmental adaptation.
- Surface proteins (the surfaceome) mediate host-pathogen interactions during infection.
- Understanding the surfaceome is key to deciphering infection mechanisms.
Purpose of the Study:
- To review the cell surface shaving technique for microbial surfaceome analysis.
- To highlight microbial proteins involved in host-pathogen interactions.
- To provide examples of identified microbial proteins and their roles.
Main Methods:
- Cell surface shaving technique to isolate and analyze surface proteins.
- Mass spectrometry for protein identification.
- Bioinformatic analysis for functional annotation.
Main Results:
- The cell surface shaving technique effectively identifies microbial surface proteins.
- Key microbial proteins involved in host cell adhesion and invasion were identified.
- Interactions between microbial and host molecules were elucidated.
Conclusions:
- The cell surface shaving technique is a powerful tool for studying microbial surfaceomes.
- Identified surface proteins offer potential targets for therapeutic interventions.
- This approach advances our understanding of host-pathogen dynamics.
Related Concept Videos
01:29Types of Microorganisms
2.2K
Microorganisms are a diverse group of microscopic entities broadly categorized into cellular and acellular types based on their structural organization. Cellular microorganisms include bacteria, archaea, fungi, protozoa, and algae, while acellular microorganisms are represented by viruses.Cellular MicroorganismsBacteriaBacteria, tiny prokaryotic organisms, exhibit fascinating shapes such as rods, spheres, and spirals. They adapt to diverse habitats, including soil, water, and human-associated...
2.2K
01:18Mutations in Microorganisms
717
Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
717
01:21Cell-surface Signaling
54.3K
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
54.3K
01:33Proteomics
9.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.7K
01:30Environmental Applications of Microorganisms
1.1K
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
1.1K
01:29Microorganisms in Medicine and Therapeutics
1.1K
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1.1K