Related Experiment Video
Updated: Jan 29, 2026

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Modification of Soybean 11S Protein by Fermentation: Antioxidant Capacity, Oxidative Stability in Emulsions and
Yaozu Guo1, Jiaxuan Han1, Boxing Yin1
1College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.
Abstract:
Fermentation is an effective method to enhance the bioactivity of plant proteins, yet the link between the functionality and conformational state of fermented soybean 11S protein (F11S) requires clarification. This study first evaluated the antioxidative efficacy of F11S and its application in emulsion systems, followed by a mechanistic investigation into its structural evolution. Results showed that the bioactivity of F11S was strictly fermentation-time-dependent, reaching its peak at 16 h. At this stage, F11S exhibited maximal scavenging capacities for ·OH (84.51 ± 2.53%) and DPPH radicals (93.84 ± 2.62%). Crucially, in a Tween 20 emulsion system, the F11S-16h fraction demonstrated superior oxidative stability, maintaining the lowest peroxide value (4.33 ± 0.53 mmol/kg) after 15 days of storage. To elucidate the mechanism behind this enhanced functionality, structural analysis was conducted. It revealed that while surface hydrophobicity peaked at 12 h due to protein unfolding, extended fermentation to 16 h induced a refolding process, guiding the protein into a thermodynamically stable conformation. These findings indicate that the stable refolded structure formed at 16 h, rather than maximal hydrophobicity, is the key determinant for the superior antioxidant performance and emulsion stabilizing ability of F11S.
Related Concept Videos
Fermentation
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Stability of structures
The Evidence for Evolution
Protein and Protein Structure
A protein's shape is critical to its function. For example, an enzyme...
Structural Protein Function
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Protein Modifications in the RER
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...

