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Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy
Published on: May 8, 2020
The Predatory Bacteria Bdellovibrio bacteriovorus LR3: A Potential Biocontrol Agent Against Gram-Negative Pathogenic
Anna P Shorokhova1, Valentina N Polivtseva1, Tatiana N Abashina1
1G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia.
Abstract:
The paper describes a predatory Gram-negative bacterium from the genus Bdellovibrio, which was isolated from water of the Lyubozhikha River. As revealed by electron microscopy, the bacterium is an intracellular predator of Gram-negative microorganisms. Its prey range includes Pseudomonas tolaasii, the phytopathogen responsible for brown spot disease in the cultivated button mushroom (Agaricus bisporus). Based on the results of a 16S rRNA gene sequence analysis, the bacterium was identified as Bdellovibrio bacteriovorus strain LR3. We characterized the predator-prey dynamics between B. bacteriovorus LR3 and P. tolaasii, determining the optimal temperature and pH conditions for this interaction. Our results demonstrate the potential of B. bacteriovorus LR3 as a biocontrol agent against P. tolaasii in mushroom cultivation. The possibility of using B. bacteriovorus LR3 against clinical cases Salmonella and Escherichia infections is also addressed.
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